Sequences from confirmation amplicon


Confirmation sequences for line SALK_016116 and hit At1g05680 are shown.
Line ID SALK_016116
Locus At1g05680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1703227
Primers used for PCR AW15 - R204, show primer details
   
Sequence Name 64-K107135-SALK_016116C-AW15
GenBank Accession LN485837 [GenBank]
Sequencing primer gene specific primer, AW15: 5'-GTTGTTTCTTGACACACTGTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K107135-SALK_016116C-AW15
CATGATTGGTATGCCACACTGGACTGATCAGCCCACGAATGCTAAGTTCATGCAGGATGT
GTGGAAGGTTGGGGTAAGGGTTAAGGCAGAAGGTGATGGGTTTGTGAGAAGAGAAGAGAT
TATGAGAAGTGTGGAAGAAGTTATGGAGGGAGAGAAAGGGAAAAAGATTAGAAAGAATGA
TGAGAAATGGAAAA
   
Sequence Name 56-K107134-SALK_016116C-R210
GenBank Accession LN485636 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107134-SALK_016116C-R210
CTCTTATCGAGCTCCTCCTTCGAAACTGCCTCTTGAGCCAACACTTTCCATTTCTCAGCA
TTCTTTCTAATCTCTTTCCCTTTCTCTCCCTCCATAACTTCTTCCACACTTCTCATAATC
TCTTCTCTTCTCACAAACCCATCACCTTCTGCCTTAACCCTTACCCCAACCTTCCACACA
TCCTGCATGAACTTAGCATTCGTGGGCTGATCAGTCCAGTGTGGCATACCAATCATTGGA
ACTCCCAAACTCAATCCCTCTAACGTCGAGTTCCATCCACAGTGTGTCAAGAAACAA

Last Updated on 06.12.2018 16:11