Sequences from confirmation amplicon


Confirmation sequences for line SALK_016240c and hit At5g54590 are shown.
Line ID SALK_016240c
Locus At5g54590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22182417
Primers used for PCR 01df - LBb3, show primer details
   
Sequence Name 09-K109155-SALK_016240C-01df
GenBank Accession LN484515 [GenBank]
Sequencing primer gene specific primer, 01df: 5'-TCACTCTTCTTCTTTTTCCTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K109155-SALK_016240C-01df
GATAAATTGGATAACGATAAACGACACTGTATTATCACTAAACCTTAATGGGCTAGGTTA
GGTTTTAATTGGGCCTTGAATTCAATTCTTTTCATAGCCCAAATATATAATATGGTTCTC
CAGTTTTGAACTCGTTTTAACACGAAAAAACAAAGAACAAATTTTCATTCACAAATATAG
ACAATCTAGCGTCTAAAAACCCAAAAGAATTGAAAAACAAAATCACATTTTAAAAGTTAA
TAATAATTAAAAAAATTGAGACTTTTTTTTCATTTTGTTTACAAAATCACGTCTTCAGCT
ATACTACTGTCCAAGGAATTATCCCTCCGATGAATTTCTGATCGTAATGATCCGTTTGCA
GTTAACTCACCTTCTGACTCCTCCACTATCGGAGGAGGAGGAGGAAGCCGTGGAGACGGC
GACGGAGAATTCTTCTGCCGCTTTCTGCAATGTCTCACTTTAATAACACGAGTCAAAACC
TGAACAATGTCCCTCATGTTAGGACGTTTTCTAGGTGCACGAGAGATGCATTTGTAAGCA
AAAGCTGCTACTTCATTCACCAGGATATATATAAAAGGGCGGAATAACAAATTGTGGCAG
CAACGCATCACCACTCGTGGATTGACGCTTAGACAACTTAATAACAAATTGACGCCAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGGTTTT
CTTTT
   
Sequence Name 01-K109154-SALK_016240C-R210
GenBank Accession LN484272 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109154-SALK_016240C-R210
GTCATTTGGCGTCATTTGTTATAAGTTGTCTAAGCGTCATCCACGAGTGGTGATGCGTTG
CTGCCACAATTTGTTATTCCGCCCTTTTATATATATCCTGGTGAATGAAGTAGCAGCTTT
TGCTTACAAATGCATCTCTCGTGCACCTAGAAAACGTCCTAACATGAGGGACATTGTTCA
GGTTTTGACTCGTGTTATTAAAGTGAGACATTGCAGAAAGCGGCAGAAGAATTCTCCGTC
GCCGTCTCCACGGCTTCCTCCTCCTCCTCCGATAGTGGAGGAGTCAGAAGGTGAGTTAAC
TGCAAACGGATCATTACGATCAGAAATTCATCGGAGGGATAATTCCTTGGACAGTAGTAT
AGCTGAAGACGTGATTTTGTAAACAAAATGAAAAAAAAGTCTCAATTTTTTTAATTATTA
TTAACTTTTAAAATGTGATTTTGTTTTTCAATTCTTTTGGGTTTTTAGACGCTAGATTGT
CTATATTTGTGAATGAAAATTTGTTCTTTGTTTTTTCGTGTTAAAACGAGTTCAAAACTG
GAGAACCATATTATATATTTGGGCTATGAAAAGAATTGAATTCAAGGCCCAATTAAAACC
TAGCCTAGCCCATTAAGGTTTAGTGATAATACAGTGTCGTTTAATCGTTTATCCAATTTT
TATCGTCATCTGTGGAACGGATAGGACGAAGGAAAAA

Last Updated on 06.12.2018 16:11