Sequences from confirmation amplicon


Confirmation sequences for line SALK_016289c and hit At5g22300 are shown.
Line ID SALK_016289c
Locus At5g22300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7381076
Primers used for PCR 01cw - LBb3, show primer details
   
Sequence Name 17-K108551-SALK_016289C-01cw
GenBank Accession LN484739 [GenBank]
Sequencing primer gene specific primer, 01cw: 5'-CTAATAAATTCTCACCAAAGCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108551-SALK_016289C-01cw
GAAACTGCAACAAACACCAAGGTGGTTCTTGTTTTTTTTTGGCCTCAAGGTGGTTCTTGT
TGCTTTGAGGGAGATGCCTCTTCCTTCTTACATGAGAGCTTGTAGCATCACCAAGATCTG
ACCAGTTCATCAAGAGACGTAGCTTGGTTGCTCCACCCGTGACTCCACAACAAGCTTCAT
TCAAGGGAGGCACAGATGCTCCAAGAGTAAGAAGTTGTAGATAAGATCATTTCAGCAACA
GTCACATGGGCTTAACTTATGAAAGGCCCAGTGACTTGGGCTTTCACATGGGTCAATCTC
ATAGCCAATAAACCCAACAATTGTTAGTGTGATCATCTACTAACAATTGAGTGTTTCAGT
CTCTACTAGTTATAGTGTTTCTTGAATCACCAAGTGTGTGTAAACTGTAAATTACATTCT
ACTTATTCATCTGTGCCATCTTGTGTTAGGCCCTGAAGTGGAACGATTAGCGTTAATGGC
CAAGAAGTACAAAGTATACTTGGTTATGGGTGTGATAGAGAGGGAAGGCTACACGCTATA
CTGCACCGTTCTTTTCTTCGATTCACAAGGTCTGTTCTTAGGTAAGCACCGCAAACTCAT
GCCTACAGCTCTTGAACGTTGCATTTGGGGATTTGGAGATGGAT
   
Sequence Name 07-K108550-SALK_016289C-R210
GenBank Accession LN484451 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108550-SALK_016289C-R210
TAGGAGTATCGAACACAGGGCATGGTTGATCCATCTCCAAATCCCCAAATGCATCGTTCA
AGAGCTGTAGGCATGAGTTTGCGGTGCTTACCTAAGAACAGACCTTGTGAATCGAAGAAA
AGAACGGTGCAGTATAGCGTGTAGCCTTCCCTCTCTATCACACCCATAACCAAGTATACT
TTGTACTTCTTGGCCATTAACGCTAATCGTTCCACTTCAGGGCCTAACACAAGATGGCAC
AGATGAATAAGTAGAATGTAATTTACAGTTTACACACACTTGGTGATTCAAGAAACACTA
TAACTAGTAGAGACTGAAACACTCAATTGTTAGTAGATGATCACACTAACAATTGTTGGG
TTTATTGGCTATGAGATTGACCCATGTGAAAGCCCAAGTCACTGGGCCTTTCATAAGTTA
AGCCCATGTGACTGTTGCTGAAATGATCTTATCTACAACTTCTTACTCTTGGAGCATCTG
TGCCTCCCTTGAATGAAGCTTGTTGTGGAGTCACGGGTGGAGCAACCAAGCTACGTCTCT
TGATGAACTGGTCAGATCTTGGTGATGCTACAAGCTCTCATGTAAGAAGGAAGAGGCATC
TCCCTCAAAGCAACAAGAACCACCTTGATACCTAAAAAAAACAAGAACCACCTTGGTTGT
TTGTTGCAGTTTCTGTTACAGTGTCGTAGTAGAACAAAAGCTGTTTTAAAGACGGCTTTG
GTGAGAATTT

Last Updated on 06.12.2018 16:11