Sequences from confirmation amplicon


Confirmation sequences for line SALK_016403c and hit At1g20370 are shown.
Line ID SALK_016403c
Locus At1g20370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7051788
Primers used for PCR 47dl - LBb3, show primer details
   
Sequence Name 61-K109940-SALK_016403C-47dl
GenBank Accession LN485760 [GenBank]
Sequencing primer gene specific primer, 47dl: 5'-TGTTGCTATCATGAGGAACTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K109940-SALK_016403C-47dl
GAGTAAGCTTTTTGTTTCNATTATTTCCCTGAATATATAATTGTTTGAGTCGTTTTGATT
TGTATTGATTATGGAATTGGTAACTCTTCACCAGGGATGTGAGAATAAATGTACCGATGG
CGCCAGAAGTTGGATTATACCTTGATGAATGCTTCTTCACGTCTTACAACAAAAGGTTTA
AAGGCAGTCATGAGGAGGTGTCAATGGAAGAGTACAAGGAAGTAGCTGAAGAGTTCAAAT
GGAAGTATGTTTATTCACATATTGGCTCTGCTGAAGAAAAAGATGGAGCTGTGGCGATTT
GGTTGCATTCTCTGAACCAGAGAAACTATCCTGATCTACGAAGCAATGAATACCAACCGG
ATGAAGTCATTGTCTATAAGAAGATTGGTGAAGCCTCTGAAGAAAATATCCATGAAGGCA
ATGCTGAGCTATTCGTTGTCGATAAAGTGAATGATGAAACCT
   
Sequence Name 52-K109939-SALK_016403C-R210
GenBank Accession LN485555 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K109939-SALK_016403C-R210
CGGGTGAGCTTGATGCTTCCCCTTAATCCTGTGATCCGCAGTAGATAAAAGCGGCTTCAC
CCTGTTGCCTTCTCCTCTAGTGTGGTCCTCTCTTCCATAGTCGTTCCTTCAGAGGTTTCA
TCATTCACCTTATCGACAACGAATAGCTCAGCATTGCCTTCATGGATATTTTCTTCAGAG
GCTTCACCAATCTTCTTATAGACAATGACTTCATCCGGTTGGTATTCATTGCTTCGTAGA
TCAGGATAGTTTCTCTGGTTCAGAGAATGCAACCAAATCGCCACAGCTCCATCTTTTTCT
TCAGCAGAGCCAATATGTGAATAAACATACTTCCATTTGAACTCTTCAGCTACTTCCTTG
TACTCTTCCATTGACACCTCCTCATGACTGCCTTTAAACCTTTTGTTGTAAGACGTGAAG
AAGCATTCATCAAGGTATAATCCAACTTCTGGCGCCATCGGTACATTTATTCTCACATCC
CTGGTGAAGAGTTACCAATTCCATAATCAATACAAATCAAAACGACTCAAACAATTATAT
ATTCCGGGGAATTATTAGAAACCAAAAGCTTACTTCT

Last Updated on 06.12.2018 16:11