Sequences from confirmation amplicon


Confirmation sequences for line SALK_016726c and hit At2g27080 are shown.
Line ID SALK_016726c
Locus At2g27080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11566906
Primers used for PCR 42lm - LBb3, show primer details
   
Sequence Name 55-K114145-SALK_016726C-R210
GenBank Accession LN485627 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114145-SALK_016726C-R210
TGTCCGCATGTGTTCTTCGCCGGCTGATAAACACCGGCATAACACCGTTACTTATATCAA
CGTCGTTGTAATACACATCAACAGAGCTTTCTTTCTCGTAGTAAACCCCGATTTTACCGT
TACCGTTACGTGATCTAACGGTAACGTTAAAACTCGGCGAAATCGGAGATGTTGAATTCA
GATTGATACCGGAAACAGAGAATCCTTCGATGGAGTATTTAGGAGCTTCGGGACGGTAGA
TTAGGTAGAGAACCGCGAAGGATATTCCGGCGAGGACGATTAAGATGAAAACCGCGGCGA
GGAATGAGCAGAAGCAGCATCTACAGTTACTGCGGTTGGTTTTTTTACGAGAGAGTTGTT
CAAAGCGGTGTGCGTTTTCCGGCGGTGGTATACGGTAGATTTGATCTTTTGGGACTTGGA
TTACGTAGTTTGATGG
   
Sequence Name 66-K114146-SALK_016726C-42lm
GenBank Accession LN485904 [GenBank]
Sequencing primer gene specific primer, 42lm: 5'-CACCATCAACATACGTAATCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114146-SALK_016726C-42lm
ACCGCCGGAAACGCAACCGCTTTGAACAACTCTCTCGTAAAAAAACCAACCGCAGTAACT
GTAGATGCTGCTTCTGCTCATTCCTCGCCGCGGTTTTCATCTTAATCGTCCTCGCCGGAA
TATCCTTCGCGGTTCTCTACCTAATCTACCGTCCCGAAGCTCCTAAATACTCCATCGAAG
GATTCTCTGTTTCCGGTATCAATCTGAATTCAACATCTCCGATTTCGCCGAGTTTTAACG
TTACCGTTAGATCACGTAACGGTAACGGTAAAATCGGGGTTTACTACGAGAAAGAAAGCT
CTGTTGATGTGTATTACAACGACGTTGATATAAGTAACGGTGTTATGCCGGTGTTTTATC
AGCCGGCGAAGAACACATTGCGGACAAATTGACGCTTAAACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11