Sequences from confirmation amplicon


Confirmation sequences for line SALK_016942c and hit At3g52270 are shown.
Line ID SALK_016942c
Locus At3g52270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19388425
Primers used for PCR 09rb - LBb3, show primer details
   
Sequence Name 66-K116055-SALK_016942C-09rb
GenBank Accession LN485907 [GenBank]
Sequencing primer gene specific primer, 09rb: 5'-CTCCAATCTCATTCTTTTCCTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K116055-SALK_016942C-09rb
CGCCAAGTCATCCTCGGCGTCGATCCTCTGGCTCACGAGGACGAAGGGACCCAAGTATGC
TTTCGTCCTGCGCATTTTCTCTCCTCTCTGCATGCGTATCTCTCGTTACATTGAATCTTC
CTCAGGAATTGCGTGGTTTAGCTATTTAGGGTTTCTAGTAACCATTCGGGAAAATTTCAT
CCTTTGAGAAACTTGCTTTTACATTCTCTGAAATTCCATGGATTTGGGGAAATGGGTTTT
CTGAGTTTGGAGATATCAAGTGGTCCTCACTTTGATCATGAAATTCCTTGTAAACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
CGAATCGGGCAAAAATAAA
   
Sequence Name 39-K116054-SALK_016942C-R210
GenBank Accession LN485281 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116054-SALK_016942C-R210
GAATTTCATGATCATAGTGAGGACCACTTGATATCTCCACACTTTAAAAACCCTTTTCCC
CAAATCCGTGGAATTTCAGAGAATGTAAAAGCAAGTTTCTCAAAGGATGAAATTTTCCCG
AATGGTTACTAGAAACCCTAAATAGCTAAACCACGCAATTCCTGAGGAAGATTCAATGTA
ACGAGAGATACGCATGCAGAGAGGAGAGAAAATGCGCAGGACGAAAGCATACTTGGGTCC
CTTCGTCCTCGTGAGCCAGAGGATCGACGCCGAGGATGACTTTGGCGTCAGGACGGTACG
GATCGTCAGGACGGTACGGATCGTCAGGAAAAGAATGAGATTGGAGAGGGTGGTTTTTCT
TTTCTTCAATAAG

Last Updated on 06.12.2018 16:11