Sequences from confirmation amplicon


Confirmation sequences for line SALK_017057 and hit At5g60760 are shown.
Line ID SALK_017057
Locus At5g60760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24439466
Primers used for PCR 26jd - LBb3, show primer details
   
Sequence Name 20-K113259-SALK_017057C-26jd
GenBank Accession LN484853 [GenBank]
Sequencing primer gene specific primer, 26jd: 5'-TTCACATCGCTTTCATTGAATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K113259-SALK_017057C-26jd
ACTTGTATCTTGTCGTTTATAGTTTGGGATTATTTTCGTGTTTTTTTTTTTTTTTTAAAA
AAAAAAAACGGGCTTTTTTTTGGGATTCCGGAACCAGGGTGGTCCCGGAAATAAAAAAAA
ACAAACCCTTTGGGCTTAACCCCCTTTTTTGGGATTTTTTCGGCCCCCCAAAGGGGCCTT
TCCCAAATTTTGGCTTGGAATTTGGAAAAAAATTTGCAAAAAACCGGGCCCCAAAAAATA
AAAATTTTTAACCGGGGGGGTCCCTTTTTCAGTTGGGAATTTCCGGGGGTTTTCTTTTGG
GGGTTTAAAAAACCGGGACCCAAAAAAGAAGGCCCTAATTTTCCTTGGGCCCCTACTTTG
GGGGGTTCAAGGGTGCCCAAAAAGGACCTGGCGGGGACCCCTTAAAGGGTAACCTTTTTT
TTAAAAAAAAAAAAAAACCCTCCTGAATTAGGGGGGGTCCAAACCCCCCCCTGGTTATTT
TGAATGACCCCAAAATCCCTTTTTTTTTTTTTTTGGTGGGGGGGGGGAAAAAATACCCCC
CGGGGGTTTGGATTTAAAAAAAGGGGGGGGTTTTTAAACCTTTCCCCCCCCCTTTCCCCC
CCCCAAAAAAAAAAATCCGAATTTCCAAAAAACGGGGAGCCCCCCCCCTGGGCCAATGGA
AAAAAGGGCAAAAAAATTAAACGTTTGGCCCCCGGAGGGTTTGAAAAAAAAAAAAAAAAC
CCCTTTGGGGGGGCCAAAAGGGCAACTTAAAAACCCCTTGGGGAACTTTTTAAGGGGCGG
GGGG
   
Sequence Name 03-K113258w-SALK_017057C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113258w-SALK_017057C-R210
CTTTGTCCCTCAACGGCTTACTTTTACTCTTATCAACATCATCAGTAGCCAAACTGTTAA
CTTTGTTAGCCTTTTCTACATTAGCACAAGCTGAAGCATCACCTGTCTTCTGAAATTCAG
ATTCTTTACTATGCGAGGGCGAAAGCGAGGGAGAAAGGATTAAAGATCCCTCACTTCTTA
TTAACTCAAACACCCTGCGGCTAATCTGTCCACAACCCAAACAAACAAACAAAAAACAGA
ACTTCAGGATCAATCAAAATCAACAAGGCATGAATCAGAACCACCATAAATCAAGAAGCT
TTGTCTTTAGTTTCAAACAAAAGCATAACCTTTAAAGGATACCAAGCAAGCTCATTAATG
ACAACATTGAAACACACAAAGTAAGAGCACAACGAAACTTAAGCTCATACTTTACTGTAT
CCTGATTCTTAAACACACAAAAGTAACAGCCATGAAATTCCAAACTAGAAAAGTGAACAC
CCTGATTAAAAATTCATACTTTACTGGATCCTGATTCTTGACAATTATTCTCCAAATTAC
AAGACAATAATTGAGAATAGACCATGTCGGTCGACAGAATAATGCCAAATAACGAGATTA
AGACCAAAGTGCTTAGTTCTATTCAATTCCAGACACATCACTGTTCCAGAATCCCAAAAA
AAAGCCAGTTCGTTACTTTAAAAAAAAAAAAAAAAACCCCAAAATTTCCCCCAACTTATA
AACCCACAAAATTCCATTTTTTAATGAAAAAAAATTCTTATTTCAG

Last Updated on 06.12.2018 16:11