Sequences from confirmation amplicon


Confirmation sequences for line SALK_017090c and hit At5g60640 are shown.
Line ID SALK_017090c
Locus At5g60640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24372664
Primers used for PCR 34dh - LBb3, show primer details
   
Sequence Name 26-K109819-SALK_017090C-34dh
GenBank Accession LN485028 [GenBank]
Sequencing primer gene specific primer, 34dh: 5'-GAGAAAGTGTTGACTTCTGGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K109819-SALK_017090C-34dh
GTTTCTCTATTCACTTTAGTATATAGTCTATGAGTCGTTTTTAAGTAACACAATTTATTA
CATTTTTGGTGAAATTCTACAGGGTGTTGAGCACGATCAGCTTAATGCTGCTTCCAAAGC
TGAAGACGATGTGAACTTTTATCAAACAGTGAATCCTGATGTTGCAAAGATGTTTCACCT
TGATCCGGAGTCTAAAAGGCCTGCTCTTGTCCTAGTTAAGAAGGAAGAGGAGAAAATTAG
CCATTTTGGTTGGTTCTCTGTAAATCTTTGTTATGCATTGATTTAGTGATCTTGTTCTTT
GGGCTTTTGCTTACTATGTTTGTGTTTAATATGCAGATGGAGAATTTGTTAAGTCTGCTC
TAGTTAGTTTTGTGTCTGCCAACAAGCTTGCTTTGGTCTCTGTTTTCACCAGAGAGACTG
CTCCGGAAATTTTTGAGAGTGCAATCAAGAAACAGGTCTGTCAATGGTTTTAGTAGGATT
TTTTGTTTTAAATTCGAAGATGGTGTAGGTCCGTTTACTAATAACACATTGCGGACAACT
AAATTGACGCTTAGACNACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAATTGCAGCAAGCGGTCCACGCT
   
Sequence Name 10-K109818-SALK_017090C-R210
GenBank Accession LN484546 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K109818-SALK_017090C-R210
GTCATTAGTTGTCCGCAATGTGTTATTAGTAAACGGACCTACACCATCTTCGAATTTAAA
ACAAAAAATCCTACTAAAACCATTGACAGACCTGTTTCTTGATTGCACTCTCAAAAATTT
CCGGAGCAGTCTCTCTGGTGAAAACAGAGACCAAAGCAAGCTTGTTGGCAGACACAAAAC
TAACTAGAGCAGACTTAACAAATTCTCCATCTGCATATTAAACACAAACATAGTAAGCAA
AAGCCCAAAGAACAAGATCACTAAATCAATGCATAACAAAGATTTACAGAGAACCAACCA
AAATGGCTAATTTTCTCCTCTTCCTTCTTAACTAGGACAAGAGCAGGCCTTTTAGACTCC
GGATCAAGGTGAAACATCTTTGCAACATCAGGATTCACTGTTTGATAAAAGTTCACATCG
TCTTCAGCTTTGGAAGCAGCATTAAGCTGATCGTGCTCAACACCCTGTAGAATTTCACCA
AAAATGTAATAAATTGTGTTACTTTAAAAACGACTCATAGACTATATACTAAAGTGAATA
GAGGAAACATACCACCAAGGAGTTCAAGTATCCCAAAACAACTTTGTTCCCAGAAGTCAC
ACCTTTCT

Last Updated on 06.12.2018 16:11