Sequences from confirmation amplicon


Confirmation sequences for line SALK_017111c and hit At1g71020 are shown.
Line ID SALK_017111c
Locus At1g71020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26792818
Primers used for PCR 28mv - LBb3, show primer details
   
Sequence Name 20-K114624-SALK_017111C-28mv
GenBank Accession LN484856 [GenBank]
Sequencing primer gene specific primer, 28mv: 5'-CTTTCTCCATTGGCTCAGATAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114624-SALK_017111C-28mv
ACGATCCATATCTCTGCATTGCTCTTCTTAACTGTAATCTTGCTAGTTCCACCTAATCAC
AAGCAAAAAAAAACCCTTAGAAACACCTCCATGGATAGGAAAACCAAATCTTCATACATT
CTACATAATGAACAAACTTTTACCTGTTCACGGACTTCGTCAGAGATATCATATCTATCA
TATGTCAAATCACCTAATGCTTTCTCCAGCTTCCATGTAACACATTGGAACTGGAATGAG
ATCCTCTTGGCTGCCCCATCCTTCAATCAAAATCACACATCAAAGTTTAGAGAGCTCCTC
AGGTATATATTAATAATGCTCTTCACTACACAAATGTCAACAATAGTATCTTCCAATCTA
AAAAAACTAAAAACAAATTCTTCCTATAAATTACGTTCCTGCCATGGCGAGACCAAATCT
TCGTGAAATCACAAATCCATTTTCAATCGTTGAGAATGGTTTAGAATGCTTACAGAGGAC
AATATTGTGGTGTAAACAAATTGACGCTAAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGATTCC
   
Sequence Name 04-K114623-SALK_017111C-R210
GenBank Accession LN484377 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114623-SALK_017111C-R210
CCACAATATTGTCCTCTGTAAGCATTCTAAACCATTCTCAACGATTGAAAATGGATTTGT
GATTTCACGAAGATTTGGTCTCGCCATGGCAGGAACGTAATTTATAGGAAGAATTTGTTT
TTAGTTTTTTTAGATTGGAAGATACTATTGTTGACATTTGTGTAGTGAAGAGCATTATTA
ATATATACATGAGGAGCTCTCTAAACTTTGATGTGTGATTTTGATTGAAGGATGGGGCAG
CCAAGAGGATCTCATTCCAGTTCCAATGTGTTACATGGAAGCTGGAGAAAGCATTAGGTG
ATTTGACATATGATAGATATGATATCTCTGACGAAGTCCGTGAACAGGTAAAAGTTTGTT
CATTATGTAGAATGTATGAAGATTTGGTTTTCCTATCCATGGAGGTGTTTCTAAGGGTTT
TTTTTTGCTTGTGATTAGGTGGAACTAGCAAGATTACAGTTAAGAAGAGCAATGCAGAGA
TATGGATCGTTAAATTCGAAGAAGTTCTCGAGTGGTTTATCTGAGCCAT

Last Updated on 06.12.2018 16:11