Sequences from confirmation amplicon


Confirmation sequences for line SALK_017267 and hit At2g45440 are shown.
Line ID SALK_017267
Locus At2g45440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18732458
Primers used for PCR 05gq - LBb3, show primer details
   
Sequence Name 20-K112129-SALK_017267C-05gq
GenBank Accession LN484849 [GenBank]
Sequencing primer gene specific primer, 05gq: 5'-ATCATCAAGAGCCTGAACATCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112129-SALK_017267C-05gq
AAACTCAAGCCTCTTAGACAGTGGCAATGGTACATATGGTAACCTAAAAACCGGCCTCGA
AACTCCAAGCTGAGCCAAAGCAGTGTTGATTCCAATCGGGTTTGGCTCGTGGAACAGCCA
AGCCATCAAAGGTAGAAGCTTTGAGTTCAAAGATGAATTCCTACCTTCAAACATCAACTT
CCTCATCAAACCCGGAACTAAATTACTAGTAACTGATATAACTCCTGTTGCTCCATAATC
CCATCTGGAATCATGACACTCATCATCATTCCCACTCCACACAACAACCCCATTCTCAGT
GTACTCTTCGATAATGATTTAAACTGACAGTTTATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 04-K112128-SALK_017267C-R210
GenBank Accession LN484367 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112128-SALK_017267C-R210
TTTGTTACACACAATATAAACTGTCAGTTTAAATCATTATCGAAGAGTACACTGAGAATG
GGGTTGTTGTGTGGAGTGGGAATGATGATGAGTGTCATGATTCCAGATGGGATTATGGAG
CAACAGGAGTTATATCAGTTACTAGTAATTTAGTTCCGGGTTTGATGAGGAAGTTGATGT
TTGAAGGTAGGAATTCATCTTTGAACTCAAAGCTTCTACCTTTGATGGCTTGGCTGTTCC
ACGAGCCAAACCCGATTGGAATCAACACTGCTTTGGCTCAGCTTGGAGTTTCGAGGCCGG
TTTTTAGGTTACCATATGTACCATTGCCACTGTCTAAGAGGCTTGAGTTTGTGAAACTGG
TGAAGGAGATTGGACGAGAGCATTTTGTGGGTGAGAAAGATGTTCAGGCTCTTGATGA

Last Updated on 06.12.2018 16:11