Sequences from confirmation amplicon


Confirmation sequences for line SALK_017433c and hit At3g10540 are shown.
Line ID SALK_017433c
Locus At3g10540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3292291
Primers used for PCR 06gq - LBb3, show primer details
   
Sequence Name 21-K112129-SALK_017433C-06gq
GenBank Accession LN484882 [GenBank]
Sequencing primer gene specific primer, 06gq: 5'-GTCACAAACTCCTCCAAAACTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112129-SALK_017433C-06gq
TTCTATTGCCGCACCAAAAAAATTCCCGTCTTGTCCTTCTCTTGAGTCAGTTTTATCTTT
TTCAGTCTCAATCAGCATCTCCTGAGAGGGACGGTTCTCCATGGAATCCAACACATGTTG
GAGATACCTCAGTCTTGCAGAACGATGGACACAATGGACTCTCTGAATCCTCAGGTTCCA
TAACTCGGCTTGCCTCCATAGACTCTTTCGATTCAAGATGGTGAGACTCTCCTAGGCTTT
TACTTCTTTTTCACGAAAACTCTCAGTTTTAAAATCTAACTTGGTTTTCTAATGTTTGAA
TCGTAATGTAACAGGCAACAGTTTCTAGAACCGGGAGAATCGGTTCTAATGATATCAGCA
GTGAAGAAGCTACAGAAAATCACAAGTAAGAAGGTGCAGCTAATACTCACCAACAAACCA
AGACTGATATACGTTGACCCATCAAAGCTTGTAGTAAAAGGGAATATTATCTGGTCTGAT
AACTCCAACGACCTCAACGTTCAAGTCTCAAGTCCTTCCCATTTCAAGATTTGCACAGTA
ATACCCAATCCAATGGCTCCCTTGTTCAGGATATATTGTGGTGTAAACAAATTGACGCTN
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 05-K112128-SALK_017433C-R210
GenBank Accession LN484399 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112128-SALK_017433C-R210
TTTGTTTCCCACAATATATCCTGAACAGGGAGCCATTGGATTGGGTATTACTGTGCAAAT
CTTGAAATGGGAAGGACTTGAGACTTGAACGTTGAGGTCGTTGGAGTTATCAGACCAGAT
AATATTCCCTTTTACTACAAGCTTTGATGGGTCAACGTATATCAGTCTTGGTTTGTTGGT
GAGTATTAGCTGCACCTTCTTACTTGTGATTTTCTGTAGCTTCTTCACTGCTGATATCAT
TAGAACCGATTCTCCCGGTTCTAGAAACTGTTGCCTGTTACATTACGATTCAAACATTAG
AAAACCAAGTTAGATTTTAAAACTGAGAGTTTTCGTGAAAAAGAAGTAAAAGCCTAGGAG
AGTCTCACCATCTTGAATCGAAAGAGTCTATGGAGGCAAGCCGAGTTATGGAACCTGAGG
ATTCAGAGAGTCCATTGTGTCCATCGTTCTGCAAGACTGAGGTATCTCCAACATGTGTTG
GATTCCATGGAGAACCGTCCCTCTCAGGAGATGCTGATTGAGACTGAAAAAGATAAAACT
GACTCANGAGAAGGACAAGACGGGAATTTTTTTGGTGCGGCAATTAGAAGTAACTAAAGA
AGGTAAATACCGCAGGATCTGGAGCTAGTTTTG

Last Updated on 06.12.2018 16:11