Sequences from confirmation amplicon


Confirmation sequences for line SALK_017577c and hit At4g18890 are shown.
Line ID SALK_017577c
Locus At4g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10352917
Primers used for PCR 03ca - LBb3, show primer details
   
Sequence Name 88-K108074-SALK_017577C-03ca
GenBank Accession LN486314 [GenBank]
Sequencing primer gene specific primer, 03ca: 5'-GGTTGGAGATGCCTTTAAGACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K108074-SALK_017577C-03ca
GACAAGTTGCATGGCAAGTAGATCGAGAGCATCACGTGACCCTCTCGATTTAACGGTCAT
GATTGGATTTTTTGCGATCCCACCAAAGGTGACCTGTCACATTTTGTAGATGCCGCAATC
TCTGCCACGTATTTTGGACTCGCAGTTATTTTAGTTAACTACGTAATTAGTTTAGCTAAA
TTTATATATTAAGTGTAGCATGATTTTATTCATAGTCTCTATTGCATGTGGATAGTCCGT
AGTTGGAAAATGCTGTCATTGAATAAGCATAAAGTTGTAAAATGATCAAATTAGTTATTG
TAGAAGCTAGATTTTATTATACTCCAAAATAGCGTCTGACTCAAAAGAATAGTTATTATA
AAAGCAGCTTGATAGTCTTGACTTTTGAATTCTCAAAATGTAAACACAAACTGTGTCGCA
TTTCGATTTGCCTGCTTTATTAAAAAAGCCTGATCACGTGTTAACACATATGGGACATCC
TTGTAAA
   
Sequence Name 79-K108073-SALK_017577C-R210
GenBank Accession LN486204 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K108073-SALK_017577C-R210
CGTCATTTGTCAGTTGTCGCAGTGTTTAGGGAGCTTGAAGTTTCCATGAATCCTTAGTCC
TGCGAAGATCTTAGCCGCAATCGCTCGTCTTCGCCGCTCTCGCCGTTTGTTGTTCTCTCT
CTCTTTCCAAGTCGGCGTTCTAGTCCCCGACGTCATTCTCAGATCACCGGAATTTGGAAA
AATAACACGACGTCGTCTCTCTGTTTTAACTGCCTCTTCTTCTAAGTGTTATCTCGTAGA
GAGGATAAAAAATCTCGCCGGTGAAAACTAATTTCGCGCTTTATTTTTGTTTTGTTCCTT
TTTCTGGTTCTGAGTTTTTTCTGGTGGAGTAATAAATGAAGAAGCACTTAACAGGGTGAA
GAAAAAAGAAGTCTGATTGTGAAAAGACGAAATTACCCTTAAAGTATGTTGTAATTTACA
AGGATGTCCCATATGTGTTAACACGTGCTCAGGCTTTTTTAATAAAGCAGGAAAATCGAA
ATGCGACACAGTTTGTGTTTACATTTTGAGAATTCAAAGGTCAAGACTATCAAGCTGCTT
TTATAATAACTTTTCTTTTGTGTCAAACACTATTTTTGTGTATAATAAAATCTAGCTTCT
ACAATAACTAATTTTATCATTTTACAACTTTATGCTTATTCAATGACAGCATTTTCCAAC
TACGGACTATCCACATCCAATAGAGACTATGAATAAAATCATGCTACACTTAATATATAA
ATTTAGCTAAACTAATTACGTAGTTAACTAAAATAACTGCGAGTCCAAAATACGTGGCAG
AGATTGCGGCATCTACAAATGTGACAGGTCACCTTTCGTGGGATCACAAAAAATCCAATC
ATGACCGTTAAATCG

Last Updated on 06.12.2018 16:11