Sequences from confirmation amplicon


Confirmation sequences for line SALK_018002c and hit At1g12380 are shown.
Line ID SALK_018002c
Locus At1g12380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4216501
Primers used for PCR 09vt - LBb3, show primer details
   
Sequence Name 74-K118240-SALK_018002C-09vt
GenBank Accession LN486119 [GenBank]
Sequencing primer gene specific primer, 09vt: 5'-ATTCCTCAGTGCTTTGGTCTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118240-SALK_018002C-09vt
ATTTCCCAATACCCTTCACCGTCTCCACAAGACCTCCTCTGCGTAGTTAGACGGCACAGC
TCCGTTCACAAGTACCGCTCTTCTGTACAAACTCGTCCCGTTTGGTAAATTCACAATCAA
ATTCACAAGACTCTCGCCGGACTCTCCTGGTTTCCAACCGTCCGATGAGATTTGGAAGAA
CATTGCGTCTCTGATTCTCGATTCAGCCTCTGCTCTGGCTTCCTCGTGCTTCAAGTCCAA
TCTCGTGGTGGCGAAATCTCTCTTGGAGATGATTGGTAAACCAACTTGTGTTAAAAAAGC
TCGAAACTTTGGATGCTCAAGACCCGAGAGAGAAACAGAGCCGCAGGACTCGAAGACCCA
ATCAGAGAGAGAATCTAAAGCTGACTCTATCTGCGATCTGGTAAGAGACTGAGTCTGCGA
CGGTTTAGGACTCTTAAGCTTCTTCACACTGTCTTCAAGCATAGCTAAAGGACCCAAGTC
ATCTTTTCCACCGGAGAGCATCAAATGCTGCGGCGGCGGAGGAGTTGAATAATGCAACTC
TCCGCCGCAGAATCTAGAAGGATCAACAACGGTTATCGGCGTGACGTGATAAGAGCCTCC
GATGGGAGGAGGGTTAAGCCGAGAAGGAATCGCGGTGGTGACAGCGCCGGAGGAGTTACG
TTTCCGGTGATGAGTTTGAGGAGAGGAAGAGGAAGAGGTAGGAGAAGAGTAACAAATTGA
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
   
Sequence Name 51-K118239-SALK_018002C-R210
GenBank Accession LN485544 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118239-SALK_018002C-R210
TCTTCTCCACCTCTTCCTCTTCCTCTCCTCAAACTCATCACCGGAAACGTAACTCCTCCG
GCGCTGTCACCACCGCGATTCCTTCTCGGCTTAACCCTCCTCCCATCGGAGGCTCTTATC
ACGTCACGCCGATAACCGTTGTTGATCCTTCTAGATTCTGCGGCGGAGAGTTGCATTATT
CAACTCCTCCGCCGCCGCAGCATTTGATGCTCTCCGGTGGAAAAGATGACTTGGGTCCTT
TAGCTATGCTTGAAGACAGTGTGAAGAAGCTTAAGAGTCCTAAACCGTCGCAGACTCAGT
CTCTTACCAGATCGCAGATAGAGTCAGCTTTAGATTCTCTCTCTGATTGGGTCTTCGAGT
CCTGCGGCTCTGTTTCTCTCTCGGGTCTTGAGCATCCAAAGTTTCGAGCTTTTTTAACAC
AAGTTGGTTTACCAATCATCTCCAAGAGAGATTTCGCCACCACGAGATTGGACTTGAAGC
ACGAGGAAGCCAGAGCAGAGGCTGAATCGAGAATCAGAGACGCAATGTTCTTCCAAATCT
CATCGGACGGTTGGAAACCAGGAGAGTCCGGCGAGAGTCTTGTGAATTTGATTGTGAATT
TACCAAACGGGACGAGTTTGTACAGAAGAGCGGTACTTGTGAACGGAGCTGTGCCGTCTA
ACTACGCAGAGGAGGTCTTGTTGGAGACGGTGAAGGGTATTTGTGGAAATTCACCTCAGA
GATGTGTTGGGATAGTCTCTGATAAGTTTAAGACCAAAGC

Last Updated on 06.12.2018 16:11