Sequences from confirmation amplicon


Confirmation sequences for line SALK_018036 and hit At1g13160 are shown.
Line ID SALK_018036
Locus At1g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4488468
Primers used for PCR 29cv - LBb3, show primer details
   
Sequence Name 20-K109252-SALK_018036C-29cv
GenBank Accession LN484842 [GenBank]
Sequencing primer gene specific primer, 29cv: 5'-GAAGAGGTGAATGACAGTGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109252-SALK_018036C-29cv
TGAGGAATAGAAGCGAAGAGAGGATGGAGAAGCTTCTGATTCTTCTGTAGAGGATAGTGG
AAACAAAGAGAAAGCTAAGGGCAAGAAGAGGAAGATTGTAGATTTTGATGCGAATCTTCT
CTCTGCTGATACAAGCCTCCGAGCACTCAAGAGATTCGCAGAAGCAAAGAACGAAAAGCC
TTCTTTTGATGAAGGTGATGGCATACTTTCGAATGAGGACTTCCGAAAAATCAAAACACT
TCAGGTATTAGAGCCCTTTCTCTTTTGTATGTCCTCTTCGTTCTGTTTCGTAAATAAGTC
CAACGTTACTGATGTTGTGTGTCGTATACAGGCAAAGAAGGAAGCAAAAATTGCATTGGC
TCGAAAAGGATTCAAGGTTCCGAATTCTGATCAGCTAAGCAAGAAGCGGGTCGATCCAGC
CAAACTTGAAGTAAGCTGTTACTATGAGAAGCTCCAATTTAGTCACAAAATGTTTGGCAT
ACTCAGTACTAAGTGATTTTAACTCTACCCACAGGCTCACATAAGGCATAAACTAACAAA
GGAACAAAGACTGGAATTAGTTAAAGCGGGTAGGGAGGACAGAGGGAAATACAAATCCAA
GGCTGCCGTCAAACAGAAGAAGACTGGAGGATCAAGCAATAAACAGAAGGAGCACAGGAA
GAATATGCCTCTTGCTGCAATAAGATCAAAGGCTGGTGAATCAAAACGAATTAAGAAGAT
GAAGAATAG
   
Sequence Name 30-K109255-SALK_018036C-R210
GenBank Accession LN485124 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109255-SALK_018036C-R210
GTCATTTGTTACACCACAATATATCCTGGATAACACATAATTATGCAAAAACTGAACCAA
AACAAAACTGTGTATAGTTTTGAGACGGAAGAATTGAGAGATTTGGTCATTTTTTCACTT
CCAAGCTTTCCTTCCTCTAAACTGGCTTCCGCTGATGCTATTCTTCATCTTCTTAATTCG
TTTTGATTTACCAGCCTTTGATCTTATTGCAGCAAGAGGCATATTCTTCCTGTGCTCCTT
CTGTTTATTGCTTGATCCTCCAGTCTTCTTCTGTTTGACGGCAGCCTTGGATTTGTATTT
CCCTCTGTCCTCCCTACCCGCTTTAACTAATTCCAGTCTTTGTTCCTTTGTTAGTTTATG
CCTTATGTGAGCCTGTGGGTAGAGTTAAAATCACTTAGTACTGAGTATGCCAAACATTTT
GTGACTAAATTGGAGCTTCTCATAGTAACAGCTTACTTCAAGTTTGGCTGGATCGACCCG
CTTCTTGCTTAGCTGATCAGAATTCGGAACCTTGAATCCTTTTCGAGCCAATGCAATTTT
TGCTTCCTTCTTTGCCTGTATACGACACACAACATCAGTAACGTTGGACTTATTTACGAA
ACAGAACGAAGAGGACATACAAAAGAGAAAGGGCTCTAATACCTGAAGTGTTTTGATTTT
TCGGAAGTCCTCATTCGAAAGTATGCCATCACCTTCATCAAAAAAAGGCTTTTCGTTCTT
TGCTTCTGCGAATCTCTTGAGTGCTCGGAGGCTTGTATCAGCAGAGAGAAGATTCGCATC
AAAATCTACA

Last Updated on 06.12.2018 16:11