Sequences from confirmation amplicon


Confirmation sequences for line SALK_018319 and hit At3g42670 are shown.
Line ID SALK_018319
Locus At3g42670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14757104
Primers used for PCR 31vi - LBb3, show primer details
   
Sequence Name 40-K117945-SALK_018319C-31vi
GenBank Accession LN485301 [GenBank]
Sequencing primer gene specific primer, 31vi: 5'-GATGGAGTTGAGAGCCATACTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117945-SALK_018319C-31vi
CGGCTGAGAAGTGACATGTCTGGTCTTTATACCTCAGCTTAAGAGAAAACTGCATTTGCA
CCAGAAAAAAGCTTTTGAGTTTCTCTGGAAGAACCTAGCCGGGTCAGTGGTTCCCGCGAT
GATGGATCCGAGTTCAGATAAGATAGGTGGGTGTGTAGTTTCTCATACTCCCGGAGCAGG
AAAAACATTTCTAATCATAGCTTTTCTCGCGAGCTACTTGAAGATATTTCCGGGTAAAAG
ACCTTTGGTTCTTGCTCCAAAAACAACTTTGTACACTTGGTACAAGGAGTTCATCAAATG
GGAGATTCCGGTCCCTGTTCACTTGCTCCATGGTCGTAGAACCTACTGTATGTCCAAAGA
GAAGACCATTCAGTTCGAGGGAATTCCAAAACCAAGCCAAGATGTTATGCATGTTCTTGA
CTGTTTAGACAAAATCCAGAAATGGCATGCACAGCCGAGTGTTCTTGTAATGGGTTACAC
ATCGTTTCTAACTTTGATGAGGGAAGATTCAAAGTTTGCTCATAGAAAGTACATGGCTAA
AGTACTGAGAGAGTCTCCTGGATTACTGGTTCTAGACGAGGGACATAACCCGAGGAGTAC
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCG
   
Sequence Name 05-K117944-SALK_018319C-R210
GenBank Accession LN484412 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117944-SALK_018319C-R210
GTCATTTGGTACTCCTCGGGTATGTCCCTCGTCTAGAACCAGTAATCCAGGAGACTCTCT
CAGTACTTTAGCCATGTACTTTCTATGAGCAAACTTTGAATCTTCCCTCATCAAAGTTAG
AAACGATGTGTAACCCATTACAAGAACACTCGGCTGTGCATGCCATTTCTGGATTTTGTC
TAAACAGTCAAGAACATGCATAACATCTTGGCTTGGTTTTGGAATTCCCTCGAACTGAAT
GGTCTTCTCTTTGGACATACAGTAGGTTCTACGACCATGGAGCAAGTGAACAGGGACCGG
AATCTCCCATTTGATGAACTCCTTGTACCAAGTGTACAAAGTTGTTTTTGGAGCAAGAAC
CAAAGGTCTTTTACCCGGAAATATCTTCAAGTAGCTCGCGAGAAAAGCTATGATTAGAAA
TGTTTTTCCTGCTCCGGGAGTATGAGAAACTACACACCCACCTATCTTATCTGAACTCGG
ATCCATCATCGCGGGAACCACTGACCCGGCTAGGTTCTTCCAGAGAAACTCAAAAGCTTT
TTTCTGGTGCAAATGCAGTTTTCTCTTAAGCTGAGGTATTAAAGACCAGACATTGTCACT
TTCTTCAGCCGATGGCATATCTGAGGAAGCAACTGGTATAGTGAAAGTATGGCTCTCAAC
TCCATC

Last Updated on 06.12.2018 16:11