Sequences from confirmation amplicon


Confirmation sequences for line SALK_018378c and hit At5g45620 are shown.
Line ID SALK_018378c
Locus At5g45620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18503171
Primers used for PCR 28um - LBb3, show primer details
   
Sequence Name 03-K117319-SALK_018378C-28um
GenBank Accession LN484350 [GenBank]
Sequencing primer gene specific primer, 28um: 5'-CGATCAAATTTTGCTAATGTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117319-SALK_018378C-28um
CTTTGATTTTCCTATCAGCTCTACTTGGGGAGAACATCTATAACTTTGGGGAACTGTTAG
CCCATCCCATTGTAAGTTTTCCTTCATCCTCGACATAAACTACTGTTATATTTTCTTGTG
TAGATAAAAGGCTTCTTGTTGATCTTTAATTGCTTGGATTTACGATAGATACTGTTTGTG
AGTTGAGACGGCTTCTAACTCTCATGTTATAGTTTTGCTTAGAATTGTAGATGGTTCTCT
TACATGTAGCATTTCTCTGATGACAGTTGAAAAGTCTCCTTGGAACAAATGTGGAATGGC
TCTACCACATTCTACAGGCATTCAACCATGGTGATTTAGTTCAGTATCAAGAACTATGCC
GTGTGCACAACGCATCCTTGATTGCTCAACCAGCACTTGTTGAGAACGAGAAGAAGCTTT
TGGAAAAGATCAACATTCTCTGCCTTATCGAGATCATCTTCAGGTAAACTTTTCTCTAGG
CAATTGTACCTCACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCG
   
Sequence Name 19-K117318-SALK_018378C-R210
GenBank Accession LN484830 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117318-SALK_018378C-R210
TTGCCTAGAGAAAGTTTACCTGAAGATGATCTCGATAAGGCAGAGAATGTTGATCTTTTC
CAAAAGCTTCTTCTCGTTCTCAACAAGTGCTGGTTGAGCAATCAAGGATGCGTTGTGCAC
ACGGCATAGTTCTTGATACTGAACTAAATCACCATGGTTGAATGCCTGTAGAATGTGGTA
GAGCCATTCCACATTTGTTCCAAGGAGACTTTTCAACTGTCATCAGAGAAATGCTACATG
TAAGAGAACCATCTACAATTCTAAGCAAAACTATAACATGAGAGTTAGAAGCCGTCTCAA
CTCACAAACAGTATCTATCGTAAATCCAAGCAATTAAAGATCAACAAGAAGCCTTTTATC
TACACAAGAAAATATAACAGTAGTTTATGTCGAGGATGAAGGAAAACTTACAATGGGATG
GGCTAACAGTTCCCCAAAGTTATAGATGTTCTCCCCAAGTAGAGCTGATAGTGACAAATC
GAAAGCCAAATCCTGAAAGACACAGATATGATCTGACATTAGCAAATTTTGATCG

Last Updated on 06.12.2018 16:11