Sequences from confirmation amplicon


Confirmation sequences for line SALK_018984c and hit At3g58700 are shown.
Line ID SALK_018984c
Locus At3g58700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21712655
Primers used for PCR 03bt - LBb3, show primer details
   
Sequence Name 90-K108074-SALK_018984C-03bt
GenBank Accession LN486332 [GenBank]
Sequencing primer gene specific primer, 03bt: 5'-GTCTGGTTTTCATATCAACCCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K108074-SALK_018984C-03bt
TACTTGGTAGGTGTTGGAACAATTGAGTGGTCAGACTCCTGTTTTCTCCAAGGGTAAGTT
ATTTTCTAAGTTGTATCAATTCATCGTTCGATCTCTGAGAAAATTTGCTGGAGATAGTTA
GTGATCTCGACTAAAACAGGGGCACGATGCTTCTGAAGTTTAGGACGATGAGTTAGTTTT
TTTGAAGGTCATTAGTTCTTACTTTTTGGTGTATGATTTTTAATTGTTACTGGCCTGGAT
CCATAGATAATCGTAGTGAGAATCAGTAGCTCTATCAATTGTTTTGTATCAATTGCTTGG
AAGATGATTGTAACTTTGTTTTGGATGAAGAGAGTTTTGACATAAATCTTAACACTTGTT
ATCTCTTTTGCAGCTCGATACACTGTTCGTTCTTTTGGTATAAGACGTAATGAGAAGATT
GCTTGCTATGTGACTGTCAGGGGAGAGAAGGCAATGCAGCTCCTTGAGAGTGGATTGAAA
GTTAAAGAATACGAACTCTTGAGAAGGAACTTCAGCGACACTGGTTGCTTTGGTTTTGGT
ATTCAAGAGCACATTGATTTGGGTATCAAGTAAGTCTCATTCTTTCTAACACATACATAG
AACAACTTTAGAGTGGCTTTTGTGTAACCAGTGATCAAACGTACGGGGTTTTTAAATATA
TATTTGATCTCAGGTATGATCCATCGACTGGAATCTACGGCATGGACTTCTTATATTGTG
GTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACT
GGGGCGGTTTTTCTTTTCACAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCTGGCC
CTGAGAGAGTTGCAGCAAGCGGGCCCCGCCTGTTTGCCCCAGCAGCGAAAATCCTGTTT
Primers used for PCR 06cl - LBb3, show primer details
   
Sequence Name 76-K108073-SALK_018984C-R210
GenBank Accession LN486146 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K108073-SALK_018984C-R210
CGTCATTTGTTACCCACAATATAAGAAGTCCATGCCGTAGATTCCAGTCGATGGATCATA
CCTGAGATCAAATATATATTTAAAAACCCCGTACGTTTGATCACTGGTTACACAAAAGCC
ACTCTAAAGTTGTTCTATGTATGTGTTAGAAAGAATGAGACTTACTTGATACCCAAATCA
ATGTGCTCTTGAATACCAAAACCAAAGCAACCAGTGTCGCTGAAGTTCCTTCTCAAGAGT
TCGTATTCTTTAACTTTCAATCCACTCTCAAGGAGCTGCATTGCCTTCTCTCCCCTGACA
GTCACATAGCAAGCAATCTTCTCATTACGTCTTATACCAAAAGAACGAACAGTGTATCGA
GCTGCAAAAGAGATAACAAGTGTTAAGATTTATGTCAAAACTCTCTTCATCCAAAACAAA
GTTACAATCATCTTCCAAGCAATTGATACAAAACAATTGATAGAGCTACTGATTCTCACT
ACGATTATCTATGGATCCAGGCCAGTAACAATTAAAAATCATACACCAAAAAGTAAGAAC
TAATGACCTTCAAAAAAACTAACTCATCGTCCTAAACTTCAGAAGCATCGTGCCCCTGTT
TTAGTCGAGATCACTAACTATCTCCAGCAAATTTTCTCAGAGATCGAACGATGAATTGAT
ACAACTTAGAAAATAACTTACCCTTGGAGAAAACAGGAGTCTGACCACTCAATTGTTCCA
ACACCTACCAATNAATCGAAGA

Last Updated on 06.12.2018 16:11