Sequences from confirmation amplicon


Confirmation sequences for line SALK_019429c and hit At2g34380 are shown.
Line ID SALK_019429c
Locus At2g34380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14514250
Primers used for PCR 16ty - LBb3, show primer details
   
Sequence Name 26-K117180-SALK_019429C-16ty
GenBank Accession LN485042 [GenBank]
Sequencing primer gene specific primer, 16ty: 5'-TCTCTGAAATCCAAACCTTGAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117180-SALK_019429C-16ty
TTATTCCACTGCTTGCTTGAGATCATGATCGAACAACGTGCAGAGTTTCGTCCTGGAGCA
GGTATTCCTGAGATTTACGATGCATCTCTGTTCCTTGAATCCAAACTTCCTTTCTTGAAA
AGGATCATATGGAACTGGAGGAAAACTTTGTTTGTCTGGATCAGCATGAGTTTGTTCATT
ATGGAATTACTTTTCGCCTTAGTTTTCTTCAGACCTCTGATCATCCCAAGAACAGGTCAG
AGAACACAACAAAGAGATGACGGGTAAACCTACAAATTAACACATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATAGAGGCAAA
AT
   
Sequence Name 05-K117179-SALK_019429C-R210
GenBank Accession LN484409 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117179-SALK_019429C-R210
TTTGTAGGTTACCCGTCATCTCTTTGTTGTGTTCTCTGACCTGTTCTTGGGATGATCAGA
GGTCTGAAGAAAACTAAGGCGAAAAGTAATTCCATAATGAACAAACTCATGCTGATCCAG
ACAAACAAAGTTTTCCTCCAGTTCCATATGATCCTTTTCAAGAAAGGAAGTTTGGATTCA
AGGAACAGAGATGCATCGTAAATCTCAGGAATACCTGCTCCAGGACGAAACTCTGCACGT
TGTTCGATCATGATCTTCAAGCAAGCAGTAGGAATAATGTCCTTTTCGACCAAGCCTTTA
AGCTTCAAGTTCAAGGTTTGGATTTCAGAGA

Last Updated on 06.12.2018 16:11