Sequences from confirmation amplicon


Confirmation sequences for line SALK_019745c and hit At5g11630 are shown.
Line ID SALK_019745c
Locus At5g11630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3740557
Primers used for PCR 32vi - LBb3, show primer details
   
Sequence Name 62-K117993-SALK_019745C-32vi
GenBank Accession LN485805 [GenBank]
Sequencing primer gene specific primer, 32vi: 5'-AATCTGTGCGGAGATTCTTTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117993-SALK_019745C-32vi
TCACAAGACGGCGTTACACGGAAGGTAAAGTTTACTTGGTTTACTGGATCGAAAATTAAA
CAATTTTAGACGTCGTGTGGTTTTTGGTATGACTGTGGGACATCATGCCGTTTGTCTGTC
ATTGACTTTTTGTTTGATAACGTCGTCGTTTTTGGTTAAACCTCGAAAAACCCCTCTCTC
TCTTTCTTGCTCTTGTTAACCTAGAAATCAAAATCTCAGCTTCACCATTCGTTTCGTTTT
TCCGGCAAAATGGCTTCACGATAACAAATTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 51-K117992-SALK_019745C-R210
GenBank Accession LN485543 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117992-SALK_019745C-R210
GTCATTTGTTACACCACAATTTGTTATCGTGAAGCCATTTTGCCGGAAAAACGAAACGAA
TGGTGAAGCTGAGATTTTGATTTCTAGGTTAACAAGAGCAAGAAAGAGAGAGAGGGGTTT
TTCGAGGTTTAACCAAAAACGACGACGTTATCAAACAAAAAGTCAATGACAGACAAACGG
CATGATGTCCCACAGTCATACCAAAAACCACACGACGTCTAAAATTGTTTAATTTTCGAT
CCAGTAAACCAAGTAAACTTTACCTTCCGTGTAACGCCGTCTTTGTGATCTCCTACACGT
CGCCGTTATTCCACACGTGTGCCAACGTTTCATACAAAGAATCTCCGCACAGATT

Last Updated on 06.12.2018 16:11