Sequences from confirmation amplicon


Confirmation sequences for line SALK_020163c and hit At1g03790 are shown.
Line ID SALK_020163c
Locus At1g03790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:955720
Primers used for PCR 47us - LBb3, show primer details
   
Sequence Name 57-K117993-SALK_020163C-47us
GenBank Accession LN485673 [GenBank]
Sequencing primer gene specific primer, 47us: 5'-GTCTCTGTCTCCACCTATGTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117993-SALK_020163C-47us
ACCGCGCCGCTTCTGCCGTTTCCGCCGCTGCAGCCGCTGGTTCAATGAACTATAAAGACG
TTTTGAGCGAGCTTGTGAATTCTTTGGATTCCATGAGCTTAGCGGAAGCGCTGCAGGCGA
GTTCCTCGTCGCCCGTTACAACTCCGGTTTCCGCTGCGGCTGCCGCGTTTGCGTCGTCCT
GCGGTCTGAGCAATCAGCGTCTCCATCTCCAGCAACAACAACCAAGTAGCCCTTTGCAAT
TTGCTCTTTCGCCTTCCACTCCAAGTTACCTAACTAACTCACCTCAGGCGAACTTCTTCA
GTGATGATTTCACTCCTCGCCGGAGACAGATGAACGATTTCACGGCGATGACGGCCGTTA
GGGAGAATACTAACATTGAGGATGTAAATAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAAT
   
Sequence Name 46-K117992-SALK_020163C-R210
GenBank Accession LN485423 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117992-SALK_020163C-R210
GTCATTTATTTCATCCTCAATGTTAGTATTCTCCCTAACGGCCGTCATCGCCGTGAAATC
GTTCATCTGTCTCCGGCGAGGAGTGAAATCATCACTGAAGAAGTTCGCCTGAGGTGAGTT
AGTTAGGTAACTTGGAGTGGAAGGCGAAAGAGCAAATTGCAAAGGGCTACTTGGTTGTTG
TTGCTGGAGATGGAGACGCTGATTGCTCAGACCGCAGGACGACGCAAACGCGGCAGCCGC
AGCGGAAACCGGAGTTGTAACGGGCGACGAGGAACTCGCCTGCAGCGCTTCCGCTAAGCT
CATGGAATCCAAAGAATTCACAAGCTCGCTCAAAACGTCTTTATAGTTCATTGAACCAGC
GGCTGCAGCGGCGGAAACGGCAGAAGCGGCGCGGTTCCTCAACCGTGAAAAAGCAGCGGC
TTTATTAGCCGGAGACATAGGTGGAGACAGAGAC

Last Updated on 06.12.2018 16:11