Sequences from confirmation amplicon


Confirmation sequences for line SALK_020314c and hit At5g54270 are shown.
Line ID SALK_020314c
Locus At5g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22039170
Primers used for PCR 30mv - LBb3, show primer details
   
Sequence Name 23-K114624-SALK_020314C-30mv
GenBank Accession LN484950 [GenBank]
Sequencing primer gene specific primer, 30mv: 5'-AATGATCTTTGGTATGGACCTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114624-SALK_020314C-30mv
TTTCGTTAACTCCGTCTTACTCACGGAGAATTCCCTGGCGATTATGGTTGGGACACCGCC
GGTTTATCCGCAGACCCTGAAGCCTTTGCCAAAAACAGAGCTCTTGAGGTTAGTCTCTAA
ATCTCACTCACTCAATGTAAAAACAGAGTAAATAACTAATCATCATTTGCAGGTGATCCA
TGGGAGATGGGCAATGTTGGGAGCTTTTGGTTGCATAACCCCTGAAGTTCTTCAAAAGTG
GGTCCGTGTGGACTTCAAAGAACCAGTCTGGTTCAAAGCCGGTTCACAAATCTTCTCCGA
AGGCGGTTTGGACTACTTAGGCAACCCAAACCTAGTCCATGCTCAGAGCATTTTAGCCGT
CCTTGGCTTCCAAGTCATCCTCATGGGTTTGGTTGAAGGTTTCCGCATCAACGGTCTTGA
TGGTGTTGGCGAAGGCAACGACTTGTACCCCGGTGGGCAATACTTTGACAGGAGATATTG
TGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTAGATGGTGGTTC
   
Sequence Name 07-K114623-SALK_020314C-R210
GenBank Accession LN484468 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114623-SALK_020314C-R210
ACCACAATATCTCCTGTCAAAGTATTGCCNCACCGGGGTACAAGTCGTTGCCTTCGCCAA
CACCATCAAGACCGTTGATGCGGAAACCTTCAACCAAACCCATGAGGATGACTTGGAAGC
CAAGGACGGCTAAAATGCTCTGAGCATGGACTAGGTTTGGGTTGCCTAAGTAGTCCAAAC
CGCCTTCGGAGAAGATTTGTGAACCGGCTTTGAACCAGACTGGTTCTTTGAAGTCCACAC
GGACCCACTTTTGAAGAACTTCAGGGGTTATGCAACCAAAAGCTCCCAACATTGCCCATC
TCCCATGGATCACCTGCAAATGATGATTAGTTATTTACTCTGTTTTTACATTGAGTGAGT
GAGATTTAGAGACTAACCTCAAGAGCTCTGTTTTTGGCAAAGGCTTCAGGGTCTGCGGAT
AAACCGGCGGTGTCCCAACCATAATCGCCAGGGAATTCTCCGGTGAGGTAAGACGGAGTT
TGAACGGAAAAGGGTCCTAAGTACTTCACTCTGTCAGGTCCAT

Last Updated on 06.12.2018 16:11