Sequences from confirmation amplicon


Confirmation sequences for line SALK_020772c and hit At5g43620 are shown.
Line ID SALK_020772c
Locus At5g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17522776
Primers used for PCR 30vd - LBb3, show primer details
   
Sequence Name 41-K117945-SALK_020772C-30vd
GenBank Accession LN485319 [GenBank]
Sequencing primer gene specific primer, 30vd: 5'-ATTGTCAAAGCTTAATCCAACAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117945-SALK_020772C-30vd
AGTTTTCTTCTCTTTCTCATTGTTAGAAGACTAAGCAAATCAGTCAATTCTTTGCTTAAA
ACAATTGGTTGATGCATCGATTGAGACACAACTGAAACCATGTTCGAATGATTCATCGGA
TTTTGAACATTCGTAAAATCAGGTGTGTTTGGTACAAAATTCGATGACCTAACAGGATAA
TTCGGAGTTGAATTTGGAACCCATTGAGGCAACGAATCTAAAGGATCAAGCTTACGATAA
GGTAATGGAAGAGGAACAATTTGGGGATTAGGGTTTCCATAAACAACAAAGGGTTTCTGA
TAATTGTTGGTGTTAGAATCATTGTTAGGTAAAGCAAAACTTGAATTAGAAGCAATCACA
TCAACACCGTAATTGTTATATGACTGAGAAGAAACCCTAGACACGTTATTATTAGTATTA
CTACCATAATGATACAGAGGATTATGACCAGATCGGAATTGCTGTTGCAGTTGCGATCTG
AAACGTTTTTGCGGCGGCGCGTACTGGGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 06-K117944-SALK_020772C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117944-SALK_020772C-R210
CTACAACTCCGACATTAAGAAGAGGGCTTGACTAAGTGCCCATAGTAGTAACTCCTTGCA
CTCCGGATACACAGCTCTTTTTTGCGGCGCAGTGTATGGCCTTTTCCTCGTCTCGATCGA
CGAA

Last Updated on 06.12.2018 16:11