Sequences from confirmation amplicon


Confirmation sequences for line SALK_020988c and hit At1g76300 are shown.
Line ID SALK_020988c
Locus At1g76300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28625986
Primers used for PCR 47cy - LBb3, show primer details
   
Sequence Name 47-K109566-SALK_020988C-47cy
GenBank Accession LN485432 [GenBank]
Sequencing primer gene specific primer, 47cy: 5'-AGGCAAACTATTTTGTGTAACGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K109566-SALK_020988C-47cy
TATGGACTACAGAAGCCAAATTGGCAATTAAATCAGAATCTGTGAAGAAGATTTGTACAT
TGCAACATCTGTTTAGAGAATCTGAGTTGTCACAACTTTTTTTTTTGAATCTAATCACCA
CTCGGCTAGTTGTCACAAGTGTAGAGGAGCTTTAATTTACAAACAGTTCTTATCTTTTTA
TTTTTTTGGGTCAACTAAACAAATCTTATCTTATCTTATACTAATAAAACTTAAGAGTAA
AAACAAAGTAACAAAAAAGGCACACAACATTCAACTAATGGGCCCAATAAACTAATGGGC
CGATCCAAAATCTAAATAAGAAGTCCATAAGAGTGTGGAGCTTTAACGAGAGAAGCTAAA
TTGTTCAAAGCTTTTTGGCTGCTAAAAAGGAAAAGGGAAGGTTCAATTAGGGTTTAGTGA
ATCGACAAAAATGAGTCGGAGTTTGGGGATTCCGGTGAAGACAACTCCCCAGGATATATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTC
   
Sequence Name 38-K109565-SALK_020988C-R210
GenBank Accession LN485261 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K109565-SALK_020988C-R210
GCGTCATTTGTTACCCACAATATATCCTGGGGAGTTGTCTTCACCGGAATCCCCAAACTC
CGACTCATCTTCGTCGATTCACTAAACCCTAATTGAACCTTCCCTTTTCCTTTTTAGCAG
CCAAAAAGCTTTGAACAATTTAGCTTCTCTCGTTAAAGCTCCACACTCTTATGGACTTCT
TATTTAGATTTTGGATCGGCCCATTAGTTTATTGGGCCCATTAGTTGAATGTTGTGTGCC
TTTTTTGTTACTTTGTTTTTACTCTTAAGTTTTATTAGTATAAGATAAGATAAGATTTGT
TTAGTTGACCCAAAAAAATAAAAAGATAAGAACTGTTTGTAAATTAAAGCTCCTCTACAC
TTGTGACAACTAGCCGAGTGGTGATTAGATTCAAAAAAAAAAGTTGTGACAACTCAAATT
CTCTAAACAGATGTTGCAATGTACAAATCTTCTTCACAGATTCTGATTTAATTGCCAATT
TGGCTTCTTGTAGTCCATATTAATCAAAGATTCCATATTAATTGTTTGGAGCGTTACACA
AAATAGTTTGC

Last Updated on 06.12.2018 16:11