Sequences from confirmation amplicon


Confirmation sequences for line SALK_021004c and hit At5g45190 are shown.
Line ID SALK_021004c
Locus At5g45190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18280239
Primers used for PCR 48us - LBb3, show primer details
   
Sequence Name 52-K117993-SALK_021004C-48us
GenBank Accession LN485567 [GenBank]
Sequencing primer gene specific primer, 48us: 5'-GTCAAGGTTGGATGGTATTGACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117993-SALK_021004C-48us
ATTTTTGCAGGACTTGGGCATGAGATTGAAAGTGTAAGTTCTCTATCACCTCATCTTAGA
TTTCTTTCTGTGCATTGTTCCTTTAACTGCTTCAAGTTGGCTTGGTTTCTTGACATTATA
GTTCGTCCAATCCGTCCCATCATCATTGTGGACAGATAATAGTTACCCCTTTACATATAG
GTTTCGTTTAATTGGAATTTGATGTGCTGCTGATGGTTTTAGGCATACGTTTGTACTGAT
GTTAGTTCTAGCACCTTTAAACGGAAAGTTGTAGAAGTTGTGTTTATTTTATCTCAATTT
TTCTTTCTTTGGCATGAATACAAGGAAGAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCCGAAATGNGCAAAATAAAAA
   
Sequence Name 41-K117992-SALK_021004C-R210
GenBank Accession LN485320 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117992-SALK_021004C-R210
GCCAAGAAGAAAATTGAGATAAAATAAACACAACTTCTACAACTTTCCGTTTAAAGGTGC
TAGAACTAACATCAGTACAAACGTATGCCTAAAACCATCAGCAGCACATCAAATTCCAAT
TAAACGAAACCTATATGTAAAGGGGTAACTATTATCTGTCCACAATGATGATGGGACGGA
TTGGACGAACTATAATGTCAAGAAACCAAGCCAACTTGAAGCAGTTAAAGGAACAATGCA
CAGAAAGAAATCTAAGATGAGGTGATAGAGAACTTACACTTTCAATCTCATGCCCAAGTC
CTGCAAAAATGTACAGTAGGATTTGCGGAGGTATGTTTCCTTCTTTAAGTCAATACCATC
CAACCTTGAC

Last Updated on 06.12.2018 16:11