Sequences from confirmation amplicon


Confirmation sequences for line SALK_021811c and hit At1g10410 are shown.
Line ID SALK_021811c
Locus At1g10410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3417715
Primers used for PCR 40cp - LBb3, show primer details
   
Sequence Name 15-K109155-SALK_021811C-40cp
GenBank Accession LN484691 [GenBank]
Sequencing primer gene specific primer, 40cp: 5'-GAGAAGGTCTCTTAGCTCTAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K109155-SALK_021811C-40cp
GAAACATCTCTAGACTTTCTTATAATGGAGAGAAGGTCATGCTTCAGGAGCCTTGTGTAA
GTGTTTATATTGCTCTTGGTTCATTGGATTACCAGCTTTATCTGAAATCGTAAACTCACA
TATTATCTCGAAACTTGTCTTAGTTTTGTCGAAAATGCAGCTGAAGAGACCGATAGCGGG
TTCTCAGGTTCCTTTCTGTCCAATTGATAAGAAAATGCTCGATTGTTGGTCAACCATTGA
TCCAAATAGTTTTCGGGTTCGTGGCAAAACATATCTCAGGTAAGCCATTGGAAAGAATAT
ACAAATTTTACTTATGATTACTACAGTAGTTTAACCATCTTAACAAGAAACTATATTTTG
TTTTTGGTTAAGGGAGAAGAAGAAAGAGTTTGCACCTAGCCATGCTGCGTACAATCCTTT
TGGTGTCGATGTTTTCCTATCGGAACACAAAATACACCATGTTGCACAATATGTCAAACT
TCCTGTTACTACCACATCCACAAAACTCCCATCTATCCTTGTTGTAAATGTTCAGGTATG
ATCTAGTTCTGCTGATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 07-K109154-SALK_021811C-R210
GenBank Accession LN484453 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109154-SALK_021811C-R210
TTTGTTTACCCACAATATCAGCAGAACTAGATCATACCTGAACATTTACAACAAGGATAG
ATGGGAGTTTTGTGGATGTGGTAGTAACAGGAAGTTTGACATATTGTGCAACATGGTGTA
TTTTGTGTTCCGATAGGAAAACATCGACACCAAAAGGATTGTACGCAGCATGGCTAGGTG
CAAACTCTTTCTTCTTCTCCCTTAACCAAAAACAAAATATAGTTTCTTGTTAAGATGGTT
AAACTACTGTAGTAATCATAAGTAAAATTTGTATATTCTTTCCAATGGCTTACCTGAGAT
ATGTTTTGCCACGAACCCGAAAACTATTTGGATCAATGGTTGACCAACAATCGAGCATTT
TCTTATCAATTGGACAGAAAGGAACCTGAGAACCCGCTATCGGTCTCTTCAGCTGCATTT
TCGACAAAACTAAGACAAGTTTCGAGATAATATGTGAGTTTACGATTTCAGATAAAGCTG
GTAATCCAATGAACCAAGAGCAATATAAACACTTACACAAGGCTCCTGAAGCATGACCTT
CTCTCCATTTATAAGAAAGTCTAAGAGATGATTTTTTCCTGGAACTGGGAGGGCTAGAGC
T

Last Updated on 06.12.2018 16:11