Sequences from confirmation amplicon


Confirmation sequences for line SALK_021893c and hit At5g25360 are shown.
Line ID SALK_021893c
Locus At5g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8800106
Primers used for PCR 35wu - LBb3, show primer details
   
Sequence Name 37-K118859-SALK_021893C-35wu
GenBank Accession LN485259 [GenBank]
Sequencing primer gene specific primer, 35wu: 5'-CGCTCTGTTTATTCGTATTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118859-SALK_021893C-35wu
GTTTGTGTCTATTATTTCGTCCTGTCCAGGATTGAACCTCTGGAACCAAACTAGGCAACA
GTGGCTTGCAAATGGAACTTCTCAAAAAAAGGCCAAAGTTCGAGAACCTACAATAAGGTT
TCATTTTCTCTTCAACATCTGCATTAATTCTTTATGAATATAGTTCTTCACTTAATTCTA
TCATGGACAAGGATAAGTGTTGAAAACAATTTGATTGCGATATTTATTAATGTGCATTCA
CAGATAAACAAACTTACAAGTTTGAGATATCATATGCTATTCAATGTCCCTTTTCAAGCA
TTTATAGTGTATACATTTTATGCATATAGAAGCTTCCACTTATATCATGACTTCAGTTGT
TAGCTCTATTAGTGCTTCCATCAATAGTTTTCTCACCTTCTAGTATTATCTTTATTATGA
AATCTTTTTGGTAATAAAGCAAAGGCACAACTTGACTTTCTCATCCACCAAACATTGTAG
TTAAAACTTCTTAATCCAAGAATAGTAGTAGGATTCAATAAATATGCATCAGCAAGTCAT
CCAAACATTGACAAGCTGCATATTCAAAAAGCTGTTACATGATGTCTTGTGATTCTTCTA
TTTAGTCCTTTAGGCGGTTTAGGTTATGTAAGATAAGGTTATTACAGTTTACAAGTCAGA
AAGTTCATAGATGATTCTTCCGATTCTTAGCCATCANAGTTCTCGCCTATTCGAACTTGC
TGAGTTTAAAATTTTGTTTACTGCAGCTGGAATGCAACATATGAGAGCTTGTTGGGGACA
CATTGCTGGGATATATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTNGGGGTGTTTTTCTTTTCACCAGTGAGACGGGCAACA
   
Sequence Name 13-K118858-SALK_021893C-R210
GenBank Accession LN484656 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118858-SALK_021893C-R210
GTCATTTGTTTACCCACAATATATCCCAGCAATGTGTCCCCAACAAGCTCTCATATGTTG
CATTCCAGCTGCAGTAAACAAAATTTTAAACTCAGCAAGTTCGAATAGGCGAGAACTTTG
ATGGCTAAGAATCGGAAGAATCATCTATGAACTTTCTGACTTGTAAACTGTAATAACCTT
ATCTTACATAACCTAAACCGCCTAAAGGACTAAATAGAAGAATCACAAGACATCATGTAA
CAGCTTTTTGAATATGCAGCTTGTCAATGTTTGGATGACTTGCTGATGCATATTTATTGA
ATCCTACTACTATTCTTGGATTAAGAAGTTTTAACTACAATGTTTGGTGGATGAGAAAGT
CAAGTTGTGCCTTTGCTTTATTACCAAAAAGATTTCATAATAAAGATAATACTAGAAGGT
GAGAAAACTATTGATGGAAGCACTAATAGAGCTAACAACTGAAGTCATGATATAAGTGGA
AGCTTCTATATGCATAAAATGTATACACTATAAATGCTTGAAAAGGGACATTGAATAGCA
TATGATATCTCAAACTTGTAAGTTTGTTTATCTGTGAATGCACATTAATAAATATCGCAA
TCAAATTGTTTTCAACACTTATCCTTGTCCATGATAGAATTAAGTGAAGAACTATATTCA
TAAAGAATTAATGCAGATGTTGAAGAGAAAATGAAACCTTATTGTAGGTTCTCGAACTTT
GGCCTTTTTTTGAGAAGTTCCATTTGCAAGCCACTGTTGCCTAGTTTGGTTCCAGAGGTT
CAATCCTGGACAGGACGAAATAATAGACACAAACTTTCTTTAGAGAAAATGACTGCACTG
AAGGACCAAATACG

Last Updated on 06.12.2018 16:11