Sequences from confirmation amplicon


Confirmation sequences for line SALK_021907 and hit At2g36220 are shown.
Line ID SALK_021907
Locus At2g36220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15193206
Primers used for PCR CF78 - R204, show primer details
   
Sequence Name 60-K107135-SALK_021907C-CF78
GenBank Accession LN485733 [GenBank]
Sequencing primer gene specific primer, CF78: 5'-TCCCTCTCTCTACACAAAGCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107135-SALK_021907C-CF78
TATGCATCGGTGGCTCCGACCACCGCAAGATCGTATGCGAAACCCTCGCCGACGACTCAA
CGATCCCACCTTATTACAACAACTCCGCCGTTAGTCCGAGTGATTTCCCACCGGAATCTT
ATTTCTTGTCAAACGATGCTCAGCTAGAGTGGCTCAGCGACAACGCCTTCTTTGATCGTA
AAGACTCACAAAAAGGAAACTCTGGGATTCTCAATTCTAATCCCAACTCGAATCCAAGCT
CGCAACGGTTCTTACTAAAATCCAAAGCGTCGATCATCGGTTTGCCTAAACCGCAGAAAA
CGTGTTTCAACGAGGCAAAGCAACGGAGACACGCGGGCAAGAACCGGGTCATCTTGAAAC
GGGTCGGGTCAAGGATAAAGACGGATATTTCGCTTCTGGAACCTTCTTCTCCTAAAGTCT
CTTGCATCGGTAGAGTGAGATCCAGAAGAGAACGCAGCCGCCGTATGCACCGTCAAAAAT
CAAGTCGGGTCGAACCGGTAAACCGGGTTAAGAAACCCGGGTTTATGGCGAGTTTCAGAG
CTATTTTCAGGATCAAAGGCGGCTGCAAAGACGTGTCAGCGCGTGAGACTCACACATTGC
GGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGC
   
Sequence Name 52-K107134-SALK_021907C-R210
GenBank Accession LN485549 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K107134-SALK_021907C-R210
GTCATTTGTCCCATGTGTGAGTCTCCGCGCTGACACGTCTTTGCAGCCGCCTTTGATCCT
GAAAATAGCTCTGAAACTCGCCATAAACCCGGGTTTCTTAACCCGGTTTACCGGTTCGAC
CCGACTTGATTTTTGACGGTGCATACGGCGGCTGCGTTCTCTTCTGGATCTCACTCTACC
GATGCAAGAGACTTTAGGAGAAGAAGGTTCCAGAAGCGAAATATCCGTCTTTATCCTTGA
CCCGACCCGTTTCAAGATGACCCGGTTCTTGCCCGCGTGTCTCCGTTGCTTTGCCTCGTT
GAAACACGTTTTCTGCGGTTTAGGCAAACCGATGATCGACGCTTTGGATTTTAGTAAGAA
CCGTTGCGAGCTTGGATTCGAGTTGGGATTAGAATTGAGAATCCCAGAGTTTCCTTTTTG
TGAGTCTTTACGATCAAAGAAGGCGTTGTCGCTGAGCCACTCTAGCTGAGCATCGTTTGA
CAAGAAATAAGATTCCGGTGGGAAATCACTCGGACTAACGGCGGAGTTGTTGTAATAAGG
TGGGATCGTTGAGTCGTCGGCGAGGGTTTCGCATACGATCTTGCGGTGGTCGGAGCCACC
GATGCATACTGAAGAAACAAAGGCTTCTATATCGACTTGTGGCATAGAGAGCTTTGT
Primers used for PCR 46xl - LBb3, show primer details
   
Sequence Name 82-K119135-SALK_021907C-46xl
GenBank Accession LN486250 [GenBank]
Sequencing primer gene specific primer, 46xl: 5'-TGTAGATGTAACAGTGGGAAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119135-SALK_021907C-46xl
ATCACAAGCAGCACTCACTCCATTGATATCGCCATTTTCACCTTTGCTCATCTTTTTTAT
TTGCATTACCCCAAACCTTAAGCTTCAATATTTGCAACATTAATCTTCAGCTTTAAACTT
TTGTGCTTCTTTCTAAACTCTTCAAGAATTTGTCAATTTTGAATATTTTTTCGATTAATC
AAGTTTATGGTTTGGTTTGACTACTACTCATCAAGGAAAATCGCATTCCAACAACACGTA
AACAGAAACAAATACATTTTGTTTGACAATAGCATATACAGATATAATAACATTTAAACT
GAACTATTTACAACGGAAACTAGAAGAATACAAAAACAGTGCGGTGGGTGTGAAATCACC
GCTTTCGCGTTGCCGTGTTTCTTTCTGAATAAAAACGTTGATCAGCTGCCACGTCATCAC
CAACGACTTTTCAAAACGAGTTCCACTCAAAAAAATCAGACAAGACAACTAAAGTACTAT
TTCTTCTACTTTCCCCCTGGGACTTAAAACACTATATAACTGGACCCCACATGTATTACA
CTTGTCATCCGCCGCGTCAACATCCTCCACCACCCAACAAATCCGCTCTTCTACCAGATG
CGAAACGCGTCATCCCACCCAAACCGGGTACCACCGGTTCTCCGCCGTCGAATACCGACT
TCTCATCAGCTTCCGCAGGAAGTCGGCTCCTGATGTCGTGTAAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGCCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
   
Sequence Name 68-K119134-SALK_021907C-R210
GenBank Accession LN485966 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K119134-SALK_021907C-R210
GTCATTTGTTTCCGACATCAGGAGCCGACTTCCTGCGGAAGCTGATGAGAAGTCGGTATT
CGACGGCGGAGAACCGGTGGTACCCGGTTTGGGTGGGATGACGCGTTTCGCATCTGGTAG
AAGAGCGGATTTGTTGGGTGGTGGAGGATGTTGACGCGGCGGATGACAAGTGTAATACAT
GTGGGGTCCAGTTATATAGTGTTTTAAGTCCCAGGGGGAAAGTAGAAGAAATAGTACTTT
AGTTGTCTTGTCTGATTTTTTTGAGTGGAACTCGTTTTGAAAAGTCGTTGGTGATGACGT
GGCAGCTGATCAACGTTTTTATTCAGAAAGAAACACGGCAACGCGAAAGCGGTGATTTCA
CACCCACCGCACTGTTTTTGTATTCTTCTAGTTTCCGTTGTAAATAGTTCAGTTTAAATG
TTATTATATCTGTATATGCTATTGTCAAACAAAATGTATTTGTTTCTGTTTACGTGTTGT
TGGAATGCGATTTTCCTTGATGAGTAGTAGTCAAACCAAACCATAAACTTGATTAATCGA
AAAAATATTCAAAATTGACAAATTCTTGAAGAGTTTAGAAAGAAGCACAAAAGTTTAAAG
CTGAAGATTAATGTTGCAAATATTGAAGCTTAAGGTTTGGGGTAATGCAAATAAAAAAGA
TGAGCAAAGGTGAAAATGGCGATATCAATGGAGTGAGTGCTGCTTGTGATGCCAAGCGAC
GACGTCTTTGTATGGAAGATTTCCCCCGAAAATGTCTAAGGCACT

Last Updated on 06.12.2018 16:11