Sequences from confirmation amplicon


Confirmation sequences for line SALK_022152 and hit At3g09680 are shown.
Line ID SALK_022152
Locus At3g09680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2969951
Primers used for PCR 49us - LBb3, show primer details
   
Sequence Name 58-K117993-SALK_022152C-49us
GenBank Accession LN485700 [GenBank]
Sequencing primer gene specific primer, 49us: 5'-CCACTGCATAATCAAACACAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117993-SALK_022152C-49us
CAAATCAGAAATGGTAAATATATATGTTTATACAAACTTACATTTTCTCAAGAACGATTC
CTTTAGCGTGAGAAGAACAAGCAAAAGGCTTCTTCCACTCGTTGCCTCGATTAGACTTCT
TGTAATGCTTGTCCGCCCACCTCTGAGTAATCCGAAGCTGCTTAAGCTTGCGACCAGCTC
CCATACCACGTGTCTTACTGAAGATATTCAAATAGAAGATAAAAGAAATTGAGAACCAAA
GGACATTTCATCAGATGAAATCAACTCACTCAGAACATATAAAACAAATTCGGAGTTGAT
AAAGAACATAAAGTTATAATCTAAATCACAAATACTTAACACAAGAGGAGAGTATAAACA
CATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTG
GTTCCGAATCGGGCCAAAAT
   
Sequence Name 47-K117992-SALK_022152C-R210
GenBank Accession LN485444 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117992-SALK_022152C-R210
GTCATGTGTTTATACTCTCCTCTTGTGTTAGTATTTGTGATTTAGATTATAACTTTATGT
TCTTTATCAACTCCGAATTTGTTTTATATGTTCTGAGTGAGTTGATTTCATCTGATGAAA
TGTCCTTTGGTTCTCAATTTCTTTTATCTTCTATTTGAATATCTTCAGTAAGACACGTGG
TATGGGAGCTGGTCGCAAGCTTAAGCAGCTTCGGATTACTCAGAGGTGGGCGGACAAGCA
TTACAAGAAGTCTAATCGAGGCAACGAGTGGAAGAAGCCTTTTGCTTGTTCTTCTCACGC
TAAAGGAATCGTTCTTGAGAAAATGTAAGTTTGTATAAACATATATATTTACCATTTCTT
GATTTTGTTAGTAGTATAGATCTTGACTTTGATGAGACTCTGTGTTTGATTATGCAGTGG

Last Updated on 06.12.2018 16:11