Sequences from confirmation amplicon


Confirmation sequences for line SALK_022192c and hit At5g18240 are shown.
Line ID SALK_022192c
Locus At5g18240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6028728
Primers used for PCR 36dh - LBb3, show primer details
   
Sequence Name 29-K109819-SALK_022192C-36dh
GenBank Accession LN485102 [GenBank]
Sequencing primer gene specific primer, 36dh: 5'-AATGTTTGGTTAAGTGGTCCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109819-SALK_022192C-36dh
ATTTCGGGACGAACATGACATTGTCACTCTTTGAGGTAACGATGGGGTCCTCTTGCCCGA
ATTCGGATAAAACCAGTTTAACCCGACCCAGATCTATGCGAACCGACCCGGTTTGAAAAA
AGGCGTCGGACAATCCCCAATCTCTCGTCGTTCGACGGGGCCATTCGTTTCTCACTTTCC
GGTTTTTCTTCGATTTGCAACGTTGTGAATGCTTACACATCTATGGGTATGTTCTTGCTT
TGTTAAAAAGCACGGAGCTTTACGTCCTTCTCTCAAGAATTTCAGTATCTTCCATGAAGT
TAGTAAATTTATCTGTATTTATATGATGAGATCATAACTACGCAAATTAATAGTGTTGTA
CATTGTTGCACAAGATGTCTCTTTTGAGTTCGACTAATAATTCAATTTTTAGACGAATCC
CTCAAGCTAACAAAGGAAATCAAAGGCTTTTCATTTCCTGTGTGATTATCATAAAAAGGT
TGTTGCACTCTTCTAAAGCTTATTTGTTCTTCTCAATTCCAGCTGAAACCATTTAAATCA
AACTGTTTAGGACGCGTTGTGCCATAGTTTTCGTCGTGTGTGTTAAGATCTAGAGCTGTT
CTTGTGTGTGTTTCAAGCTTGCAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCCGAAATCCTGTTTGATGGTGGTT
   
Sequence Name 13-K109818-SALK_022192C-R210
GenBank Accession LN484635 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109818-SALK_022192C-R210
GTCATTTGTTTGCAGCTTGAACAACACAAGAACAGCTCTAGATCTTAACACACACGACGA
AAACTATGGCACAACGCGTCCTAAACAGTTTGATTTAAATGGTTTCAGCTGGAATTGAGA
AGAACAAATAAGCTTTAGAAGAGTGCAACAACCTTTTTATGATAATCACACAGGAAATGA
AAAGCCTTTGATTTCCTTTGTTAGCTTGAGGGATTCGTCTAAAAATTGAATTATTAGTCG
AACTCAAAAGAGACATCTTGTGCAACAATGTACAACACTATTAATTTGCGTAGTTATGAT
CTCATCATATAAATACAGATAAATTTACTAACTTCATGGAAGATACTGAAATTCTTGAGA
GAAGGACGTAAAGCTCCGTGCTTTTTAACAAAGCAAGAACATACCCATAGATGTGTAAGC
ATTCACAACGTTGCAAATCGAAGAAAAACCGGAAAGTGAGAAACGAATGGCCCCGTCGAA
CGACGAGAGATTGGGGATTGTCCGACGCCTTTTTTCAAACCGGGTCGGTTCGCATAGATC
TGGGTCGGGTTAAACTGGTTTTATCCGAATTCGGGCAAGAGGACCCCATCTGTTACCTCA
AAGAGTGACAATGTCATGTTTCGTCCCTGAAATTTTAGTAGGCGCTTATTTTCACCCATT
ATAGTTTTCTTTGGACCAC

Last Updated on 06.12.2018 16:11