Sequences from confirmation amplicon


Confirmation sequences for line SALK_022827c and hit At1g32690 are shown.
Line ID SALK_022827c
Locus At1g32690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11821651
Primers used for PCR 29um - LBb3, show primer details
   
Sequence Name 05-K117319-SALK_022827C-29um
GenBank Accession LN484410 [GenBank]
Sequencing primer gene specific primer, 29um: 5'-TATACAATGGAGACCGTTGTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117319-SALK_022827C-29um
ATCGGACTCGGAAATTCCAGCCTTTACCCTTCCCGGTCGCCGGAACCGGAACCGGAGAAA
ATCCAAATTCTCCGGCGACCGCAACGGATCTTGATTTCTTCATAATAGCCGGTTTAGTAT
CTCCTCTGTTTCCCATAAAAATTCTCCAAAACGACGACGTTTTCTTACACCTACCATGAT
CCAAACCGGAATCAATAACCGAATCCGGTTTAATCGGGATCGCCATAGATTTCGATCTCC
GAAACGCCGGTTCACATTCAATCAAACTCGCGACTCGGCCCACCGATCCAACGCCGGATA
TATTAAAACTGATATCTCCGGCGAAGATAGAGAACGACGAGGAAGACGAAGAAGACGAAG
GAGAGAAAGATACGGCGGCGCGTGAGCTACACGAACAAGGAAGATCGTGAGCACAATCTG
GACAAAGAGAAGAGAGACGTTCGTGGAGACAAAATGGACAAACGCCCCCACCGCTAAAAT
AACCGCGTTTTGAAAAGTGTTTGACAACTTGTTCCTGTACCTTCAATTTGTTTTCATTTG
TGTACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTC
   
Sequence Name 21-K117318-SALK_022827C-R210
GenBank Accession LN484894 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117318-SALK_022827C-R210
ATTGAAGGTACAGGAACAAGTTGTCAAACACTTTTCAAAACGCGGTTATTTTAGCGGTGG
GGGCGTTTGTCCATTTTGTCTCCACGAACGTCTCTCTTCTCTTTGTCCAGATTGTGCTCA
CGATCTTCCTTGTTCGTGTAGCTCACGCGCCGCCGTATCTTTCTCTCCTTCGTCTTCTTC
GTCTTCCTCGTCGTTCTCTATCTTCGCCGGAGATATCAGTTTTAATATATCCGGCGTTGG
ATCGGTGGGCCGAGTCGCGAGTTTGATTGAATGTGAACCGGCGTTTCGGAGATCGAAATC
TATGGCGATCCCGATTAAACCGGATTCGGTTATTGATTCCGGTTTGGATCATGGTAGGTG
TAAGAAAACGTCGTCGTTTTGGAGAATTTTTATGGGAAACAGAGGAGATACTAAACCGGC
TATTATGAAGAAATCAAGATCCGTTGCGGTCGCCGGAGAATTTGGATTTTCTCCGGTTCC
GGTTCCGGCGACCGGGAAGGGTAAAGGCTGGAATTTTCCGAGTCCGATCAAGGTTTTCCG
GCAATCTCGAGTCTCGAAGATGATATTTCAACAACGGTCTCCATTGTAT

Last Updated on 06.12.2018 16:11