Sequences from confirmation amplicon


Confirmation sequences for line SALK_022993c and hit At1g74080 are shown.
Line ID SALK_022993c
Locus At1g74080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27856574
Primers used for PCR 26mh - LBb3, show primer details
   
Sequence Name 78-K114279-SALK_022993C-26mh
GenBank Accession LN486192 [GenBank]
Sequencing primer gene specific primer, 26mh: 5'-TCAAACATCTTGATGAAAATCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K114279-SALK_022993C-26mh
AACACGGGTTCGGGGGAGTCAGAGAACGTGGATGAGGATAGAGATGTTGCATCAACGGTT
ATGCTACGGTTGATGGGGAGATTCGAAGAGGTATGTGTGGAGGAAGAACTCGTTGACCTC
TCACCTTCTGAAACACTTGTAGTTGGAGTAGTGTGACTAGTAAGTAGGCCATTACTTCCA
ATAATATCAGACAGAACATTGTGGTTGATTCTATGACCGAATCTGTTTGCTACTCTGTTC
AAAAACCGAGCTGATGATGATCCTGACAACTTTTTGTTATTTGAATTCTGATCATCTTTG
TCGTCATGAACGCTGCTTTTCTCGGGATGCGCGGAATGGTCAGATGATTTACCGGCTCCA
TCGAGAAGGGATTTGTGGGTCAACGGATCAATACCTTTCTTGACCAGACATTTCTTGATG
TGAGTGTTCCAATGGTTCTTGATCTCATTGTCTGTTCTTCTTGGTATTTTACGAGCTATG
GCCGACCATCTAATCAACAAAACATTATTTATTAGCAAATGTGTATATTTACATACCAAA
TCCTCACTAGATACCATTACTTTAGGAATACACATTTTGTGGATTATTAAAGGATATTTT
TTTTCTACCACATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGG
   
Sequence Name 64-K114278-SALK_022993C-R210
GenBank Accession LN485852 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K114278-SALK_022993C-R210
ACCCACAATGTGGTAGAAAAAAAATATCCTTTAATAATCCACAAAATGTGTATTCCTAAA
GTAATGGTATCTAGTGAGGATTTGGTATGTAAATATACACATTTGCTAATAAATAATGTT
TTGTTGATTAGATGGTCGGCCATAGCTCGTAAAATACCAAGAAGAACAGACAATGAGATC
AAGAACCATTGGAACACTCACATCAAGAAATGTCTGGTCAAGAAAGGTATTGATCCGTTG
ACCCACAAATCCCTTCTCGATGGAGCCGGTAAATCATCTGACCATTCCGCGCATCCCGAG
AAAAGCAGCGTTCATGACGACAAAGATGATCAGAATTCAAATAACAAAAAGTTGTCAGGA
TCATCATCAGCTCGGTTTTTGAACAGAGTAGCAAACAGATTCGGTCATAGAATCAACCAC
AATGTTCTGTCTGATATTATTGGAAGTAATGGCCTACTTACTAGTCACACTACTCCAACT
ACAAGTGTTTCAGAAGGTGAGAGGTCAACGAGTTCTTCCTCCACACATACCTCTTCGAAT
CTCCCCATCAACCGTAGCATAACCGTTGATGCAACATCTCTATCCTCATCCACGTTCTCT
GACTCCCCCGACCCGTGTTTATACGAGGAAATAGTCGGTGACATTGAAGATATGACGAGA
TTTT

Last Updated on 06.12.2018 16:11