Sequences from confirmation amplicon


Confirmation sequences for line SALK_023159c and hit At1g80300 are shown.
Line ID SALK_023159c
Locus At1g80300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30192936
Primers used for PCR 50us - LBb3, show primer details
   
Sequence Name 02-K117668-SALK_023159C-R210
GenBank Accession LN484319 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117668-SALK_023159C-R210
CTCTTTGCGATCACAACTGTGGATGAAGCCAAGAAATTCTATCCTTTGTTCGGACTTGGA
GCCAATGTTGCACTGATTTTCTCAGGAAGAACCGTGAAATACTTCTCTAACTTGAGAAAG
AATCTTGGTCCTGGAGTTGACGGCTGGGCAGTTTCGTTGAAAGCCATGATGAGCATTGTG
GTGGGAATGGGACTCGCCATTTGTCTCCTCTATTGGTGGGTCAATAGATATGTTCCTCTT
CCAACCCGTAGCAAGAACAAGAAGGTATGGTCTGCAGAAATTTGTGTTCTGTTTTTTTCA
CTTTATGACTCTTCTTCTTCTGATTTATTGTCTCTCTGCTACTCACTTTACAGGAGAAAC
CGAAGATGGGAACGATGGAAAGCTTGAAGTTCTTGGTAT
   
Sequence Name 39-K117669-SALK_023159C-50us
GenBank Accession LN485285 [GenBank]
Sequencing primer gene specific primer, 50us: 5'-ATACCAAGAACTTCAAGCTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117669-SALK_023159C-50us
CAGAGAGACATAAATCAGAAGAAGAAGAGTCATAAAGTGAAAAAAACAGAACACAAATTT
CTGCAGACCATACCTTCTTGTTCTTGCTACGGGTTGGAAGAGGAACATATCTATTGACCC
ACCAATAGAGGAGACAAATGGCGAGTCCCATTCCCACCACAATGCTCATCATGGCTTTCA
ACGAAACTGCCCAGCCGTCAACTCCAGGACCAAGATTCTTTCTCAAGTTAGAGAAGTATT
TCACGGTTCTTCCTGAGAAAATCAGTGCAACATTGGCTCCAAGTCCGAACAAAGGATAGA
ATTTCTTGGCTTCATCCACAGTTGTGATCTGCAAAGAGATAGAAGCAATGCTTTAAAACT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11