Sequences from confirmation amplicon


Confirmation sequences for line SALK_023188 and hit At3g06140 are shown.
Line ID SALK_023188
Locus At3g06140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1858214
Primers used for PCR 10vt - LBb3, show primer details
   
Sequence Name 69-K118240-SALK_023188C-10vt
GenBank Accession LN485990 [GenBank]
Sequencing primer gene specific primer, 10vt: 5'-GCGGATACACATCTTCTTCTAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118240-SALK_023188C-10vt
GCTGCAGAAACTTCTGTCCAGGACCTTTCTGAAAATGGAATCTTGTGGGTGAATAAACCT
CTGGAAACTGTGGGATAATGGTGCAATTTGGTTCCTCCTTTGCAAAGAAGGTGATAGTGA
AACTGAAAACGAAAGGACAACACAATGAGAGTCTATTCTTAAGTAACATTGATCAATACA
AATCATATAAGTGAGGTTAATCCAATTCTGAAGAAAGATGGGATCAGTTTACAAGGACAC
CTCTCTACGTTTCTGATCTAAAGTTGAGTTTAAAGCTTACACAACACATTGTAGATCAAG
CCTTGAAATCATTATTCACAAACCCATCAAGCAAATCATGTAAAGTTTAAACCTTTTCTA
TAGGGAAGAGTGATTCCAGATTGTTCTCATGAGTAAAGATCTTTCCTTTAATAGTTCCCT
AAGCCTTAATTTACCTATTTCGTCCCAATTATGGTTCTAAGCCTGATCTTAACGATCATA
TTTCTCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 46-K118239-SALK_023188C-R210
GenBank Accession LN485424 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118239-SALK_023188C-R210
ACCACAATATATCCTGGAGAATATGATCGTTAAGATCAGGCTTAGAACCATAATTGGGAC
GAAATAGGTAAATTAAGGCTTAGGGAACTATTAAAGGAAAGATCTTTACTCATGAGAACA
ATCTGGAATCACTCTTCCCTATAGAAAAGGTTTAAACTTTACATGATTTGCTTGATGGGT
TTGTGAATAATGATTTCAAGGCTTGATCTACAATGTGTTGTGTAAGCTTTAAACTCAACT
TTAGATCAGAAACGTAGAGAGGTGTCCTTGTAAACTGATCCCATCTTTCTTCAGAATTGG
ATTAACCTCACTTATATGATTTGTATTGATCAATGTTACTTAAGAATAGACTCTCATTGT
GTTGTCCTTTCGTTTTCAGTTTCACTATCACCTTCTTTGCAAAGGAGGAACCAAATTGCA
CCATTATCCCACAGTTTCCAGAGGTTTATTCACCCACAAGATTCCATTTTCAGAAAGGTC
CTGGACAGAAGTTTCTGCAGCCTTCAGGGACAGGAACTGACCTGAGCTTCTTTGTTCTTG
ATGATCTGTCTAAACCCTTAGAAGAAGATGGTTATCCGC

Last Updated on 06.12.2018 16:11