Sequences from confirmation amplicon


Confirmation sequences for line SALK_023251c and hit At3g21160 are shown.
Line ID SALK_023251c
Locus At3g21160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7417271
Primers used for PCR 43ai - R204, show primer details
   
Sequence Name 69-K106742-SALK_023251C-R210
GenBank Accession LN485970 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K106742-SALK_023251C-R210
CATTTGTCAGAAGCCAGAGAGTGAGTAGATTTCTTCCATCCTAGCCTTTTCTATGAAGAT
TCTTTCTGGCTTGACTTGTTCTAAACAGGCTTGTCTCATCTTATTGAGAAAAATATAAGT
AGTGAAGAAAATGAAATGGAAAAGGCTATTGAATCGTCTTTGTAATGTATCTGTTGTGCA
ATGTTCACTTTTAAACTGTTTCAAGATTATATAGAAAACATTAAATATTTCTGGCTAAAA
CTGCAACCTCCTATAAATAATATTGTTTTCTTCTCCATTAACTGAAGTTCGATTCCCAAC
TTTTCCTGTTCATCTTTCCCAGCCTTCATTTACTTATTGTCTTTCATTTTCAGGTGGGTA
GCAAGCTCATTAGATTTCGACAAGGATTATGCTGCCAGTATGTTTGAGACAACCATAAGG
TTGCATTTAACAGGTTTACCATGTTTTTTTTCTACATGTACTAAGAAGAGTCATCTTGTT
TAGGTTATCTGTTGTGTGTAGTTAACAAAATATGTGGTATGGAAGTGTGGCATCTGTGCT
TATATATTTTCTCTTTGTTGGCTTTCACAGTGGTAGGAGGGCTTCTCAGT
   
Sequence Name 85-K106743-SALK_023251C-43ai
GenBank Accession LN486282 [GenBank]
Sequencing primer gene specific primer, 43ai: 5'-GGCGACTATCTCGATAAAGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K106743-SALK_023251C-43ai
GAGAGAAATGTATATCCATGCCTAAAGCTTTGAAAAGATCGACAAGGAATATGACAGATA
GGACTTACCCCCGCCCATGTAGGATTGTGAGCATTTCCATGCTTCAAGTTGATAATATTA
TATGGTATACCCGATTGAGTGTCCCATGCAGGCAATAATCTGTCTGCAATATCCATAGCC
TTTTCAAGGAAAATTTTATCCCCAGAAAGATCATACGCACTGAGAAGCCCTCCTACCACT
CTGAAAGCCAACAAAGAGAAAATATATAAGCACAGATGCCACACTTCCATACCACATATT
TTGTTAACTACACACAACAGATAACCTAAACAAGATGACTCTTCTTAGTACATGTAGAAA
AAAAACATGGTAAACCTGTTAAATGCAACCTTATGGTTGTCTCAAACATACTGGCAGCAT
AATCCTTGTCGAAATCTAATGAGCTTGCTACCCACCTGAAAATGAAAGACAATAAGTAAA
TGAAGGCTGGGAAAGATGAACAGGAAAAGTTGGGAATCGAACTTCAGTTAATGGAGAAGA
AAACAATATTATTTATAGGAGGTTGCAGTTTTAGCCAGAAATATTTAATGTTTTCTATAT
AATCTTGAAACAGTTTAAAAGTGAACATTGCACAACAGATACATTACAAAGACGATTCAA
TAGCCTTTTCCATTTCATTTTCTTCACTACTTATATTTTTCTCAATAAGAT

Last Updated on 06.12.2018 16:11