Sequences from confirmation amplicon


Confirmation sequences for line SALK_023637 and hit At2g32290 are shown.
Line ID SALK_023637
Locus At2g32290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13715994
Primers used for PCR 74by - LBb3, show primer details
   
Sequence Name 31-K108287-SALK_023637C-74by
GenBank Accession LN485142 [GenBank]
Sequencing primer gene specific primer, 74by: 5'-TGACCAACTATGTTCCTGTCTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K108287-SALK_023637C-74by
GACGATAACGTTCTTGAAACGAAGAGAGTCTCAAGAAACAGCTCAAGAAACTGAAACAAA
GTCAAGTAGATGGAGTGATGGTTGACGTATGGTGGGGAATCGTTGAATCAAAAGGTCCAA
AGCAATATCAATGGAGTGCTTATAGAAACTTGTTTGCGATCGTACAAAGCTTTGGACTAA
AGTTACAAGCTATAATGTCGTTTCATAGATGTGGAGGCAACATAGGAGACGATGTCAACA
TCCCGATACCGAAATGGGTACTCGAAATCGGGGATTCGAATCCGGATATCTTCTATACGA
ACAAGAGTGGTAATAGAAACAAAGAGTGTTTGTCTCTCTCTGTAGATAACTTGTCTCTCT
TCAGAGGACGAACAGCTGTTGAGGTATGAAAAACTCTTGATCTTAGAACGTAACTCGGAA
AAAATTGCGTTTATATATTGATATAATCGTTGCTCTGTTTCTAGATGTATAGAGACTACA
TGAAGAGCTTTAGAGAGAACATGGAAGATTTCATAAGCTCTGGAGTTATAATAGACATTG
AAGTAGGGCTTGGTCCTCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCCGGCAAAA
   
Sequence Name 15-K108286-SALK_023637C-R210
GenBank Accession LN484688 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K108286-SALK_023637C-R210
GCGTCATTTGTTTACCCACATATATCCTGGAGGACCAGCCCTACTTCAATGTCTATTATT
ACTCCAGAGCTTATGAAATCTTCCATGTTCTCTCTAAAGCTCTTCATGTAGTCTCTATAC
ATCTAGAAACAGAGCAACGATTATATCAATATATAAACGCAATTTTTTCCGAGTTACGTT
CTAAGATCAAGAGTTTTTCATACCTCAACAGCTGTTCGTCCTCTGAAGAGAGACAAGTTA
TCTACAGAGAGAGACAAACACTCTTTGTTTCTATTACCACTCTTGTTCGTATAGAAGATA
TCCGGATTCGAATCCCCGATTTCGAGTACCCATTTCGGTATCGGGATGTTGACATCGTCT
CCTATGTTGCCTCCACATCTATGAAACGACATTATAGCTTGTAACTTTAGTCCAAAGCTT
TGTACGATCGCAAACAAGTTTCTATAAGCACTCCATTGATATTGCTTTGGACCTTTTGAT
TCAACGATTCCCCACCATACGTCAACCATCACTCCATCTACTTGACTTTGTTTCAGTTTC
TTGAGCTGTTTCTTGAGACTCTCTTCGTTTTCAAGAACGTTATCGTTCGTGATGACTCCA
AGCTGAAGCATGACGTAGACAGGAACATAGTTGGTC

Last Updated on 06.12.2018 16:11