Sequences from confirmation amplicon


Confirmation sequences for line SALK_023915c and hit At4g13940 are shown.
Line ID SALK_023915c
Locus At4g13940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8056278
Primers used for PCR CE24 - R212, show primer details
   
Sequence Name 09-K107118-SALK_023915C-R214
GenBank Accession LN484510 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K107118-SALK_023915C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTGTCCGCAATTTGTTTACACCGCAATGTGTAATGTGTTACC
CTGGTGTGAAGCGTATCACCATCAAGCCACAGACTGACAGGTGGGTGTTCCCAGAGACCA
AGGCTGGAATCATTGTCTTGGCTGAGGGTCGTCTGATGAACTTGGGTTGTGCCACTGGTC
ACCCAAGTTTCGTGATGTCTTGCTCTTTCACCAACCAGGTGATTGCCCAGCTCGAGCTCT
GGAACGAGAAAGCAAGCGGAAAGTACGAGAAGAAGGTGTACGTTCTTCCCAAGCATTTGG
ATGAGAAGTTTGCATTA
   
Sequence Name 25-K107119-SALK_023915C-CE24
GenBank Accession LN484993 [GenBank]
Sequencing primer gene specific primer, CE24: 5'-GTAATGCAACCTTCTCATCCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K107119-SALK_023915C-CE24
TTTCGCTTGCTTTCTCGTTCCAGAGCTCGAGCTGGGCAATCACCTGGGTTGGTGAAAGAG
CAAGACATCACGAAACTTGGGTGACCAGTGGCACAACCCAAGTTCATCAGACGACCCTCA
GCCAAGACAATGATTCCAGCCTTGGTCTCTGGGAACACCCACCTGTCAGTCTGTGGCTTG
ATGGTGATACGCTTCACACCAGGGTAACACATTACACATTGCGGTGTAAACAAATTGCGG
ACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGAATTAAG
GGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGA
CAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAG
CACATACGTCAGAAACCATTATTGCGCGTTCAAATCGNCCCTAAGG
Primers used for PCR 68xo - LBb3, show primer details
   
Sequence Name 73-K119300-SALK_023915C-68xo
GenBank Accession LN486095 [GenBank]
Sequencing primer gene specific primer, 68xo: 5'-ACTCTTTTGATTCATGAGGGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K119300-SALK_023915C-68xo
TCAGTTCCTGATCCTACTTCTACTGATAACCCTGAGTTTCAGATCGTGTTGTCTATTATC
AAGGAAGGTCTTCAAGTTGATCCTAAGAAGTACCACAAGATGAAGGAGAGACTTGTTGGT
GTCTCTGAGGAAACTACCACTGGTGTTAAGAGGCTTTACCAGATGCAGCAAAATGGAACT
CTTTTGTTCCCTGCCATTAACGTTAACGACTCTGTCACCAAGAGCAAGGTATTGATCTTC
AGATTTGTTTCTTTACTAGTGATGATAAATCTGCTAGGTCTTTAGCTCTGTAGTTTTTGA
TGCGATAGGCTTAACAACAACATAATCACTAAGTAGAAGCAAGCTTTTCATTGTTGGTTG
TTGAAAGTATTACGATCTAGTAGAGAAAAGTCCTTCCTTGGTTACAGAATTTGCATGAAA
AGTCAATATTTCTCATGCTTTCTTTGATTTTATATTCAACGTTTTGTTTACACTTATGTG
CTGTTACTGATAAATTCATTTGTTCTTGATTTACAGTTCGACAACTTGTATGGTTGCCGT
CACTCACTCCCTGATGGTCTCATGAGGGCCACTGATGTCATGATCGCTGGAAAGGTTGCT
GTTATCTGTGGATATGGTGATGTTGGAAAGGGTTGTGCTGCTGCCATGAAGACTGCTGGT
GCTAGAGTCATTGTGACTGAGATTGATCCCATCTGTGCCCTTCAAGCTTTGATGGAAGGA
CTTCAGGTTCTTACCCTTGAGGATGTTGTCTCAGAAGCTGATATCTTTGTCACCACCACC
GGTAACAAAGACATCATCATGGTCGACCACATGAGGAAGATGAAGAACAACGCTATTGTG
TGCCACATTGGTCACTTTGACAATG
   
Sequence Name 25-K119299-SALK_023915C-R210
GenBank Accession LN485020 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119299-SALK_023915C-R210
TCTCATTGTCAAGTGACCAATGTTGCACACAATAGCGTTGTTCTTCATCTTCCTCATGTG
GTCGACCATGATGATGTCTTTGGTTACCGGTGGTGGTGACAAATATATACAGCTTCTGAG
ACAACATCCTCAAGGGTAAGAACCTGAAGTCCTTGCATCAAAGCTTTTAAGGAAGAGATG
GGATCAATCTCACTCACAATGACTCTAGCACCAGCAGTCTTCATGGCAGCAGCACAACCC
TTTCCAACATCACCATATCCACATATAACAGCAACCTTTTCAGCGATCATGACATCAGTG
GCCCTCATGAGACCATCATGGAGTGAGTGACGGCAACCATGACGAGTTGTCCAACTGGAA
ATCAAAAACAAATTGAA

Last Updated on 06.12.2018 16:11