Sequences from confirmation amplicon


Confirmation sequences for line SALK_024241c and hit At1g33060 are shown.
Line ID SALK_024241c
Locus At1g33060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11978007
Primers used for PCR CF50 - R204, show primer details
   
Sequence Name 03-K107313-SALK_024241C-CF50
GenBank Accession LN484326 [GenBank]
Sequencing primer gene specific primer, CF50: 5'-TCAAAGGTCTGTCTCTTCAGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107313-SALK_024241C-CF50
AACTTTCCGGAGATGACGACGGAGCAAGCTTTGTTGTCTATGGAAGCTTTACCTTTAGGT
TTCAGATTCAGACCAACGGATGAAGAACTCATCAATCATTACCTAAGGTTAAAAATCAAC
GGCCGTGATTTAGAGGTTAGAGTCATCCCTGAGATCGATGTTTGCAAGTGGGAACCATGG
GACTTACCTGGTTAGTCTCTTCTTCTTCATGATTTCATTTTTGAGAATTGAAAGGTGGAA
GCTTTGTGAATTTTGGATTGTTATTGAGAGTGATGATGATAAGATTTTTTAGGGTTTTGT
TTGAATGTTTAGGGCTATCGGTGATAAAGACAGATGATCAAGAATGGTTCTTTTTTTGTC
CTCGTGATCGAAAGTATCCGAGTGGTCATCGTTCTAATAGAGCTACTGATATTGGTTACT
GGAAAGCTACTGGGAAAGATCGAACTATTAAGTCTAAGAAGATGATTATTGGTCTTCACC
CAACCTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACG
   
Sequence Name 43-K107289-SALK_024241C-R210
GenBank Accession LN485347 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107289-SALK_024241C-R210
ATCATCTTCTTGACTTAATAGTTCGATCTTTCCCAGTAGCTTTCCAGCTAACTCAATATC
AGTAGCTCTATTAGAACGATGACCACTCGGATACTTTCGATCACGAGGACAAAAAAAGAA
CCATTCTTGATCATCTGTCTTTATCACCGATAGCCCTAAACATTCAAACAAAACCCTAAA
AAATCTTATCATCATCACTCTCAATAACAATCCAAAATTCACAAAGCTTCCACCTTTCAA
TTCTCAAAAATGAAATCATGAAGAAGAAGAGACTAACCAGGTAAGTCCCATGGTTCCCAC
TTGCAAACATCGATCTCAGGGATGACTCTAACCTCTAAATCACGGCCGTTGATTTTTAAC
CTTAGGTAATGATTGATGAGTTCTTCATCCGTTGGTCTGAATCTGAAACCTAAAGGTAAA
GCTTCCATAGACAACAAAGCTTGCTCCGTCGTCATCTCCGGTAAAGTTTTGTTTTTTATT
TGGTTCATAAACAAAACCTGAAGAGACAGACCTTTGA

Last Updated on 06.12.2018 16:11