Sequences from confirmation amplicon


Confirmation sequences for line SALK_024296 and hit At3g56580 are shown.
Line ID SALK_024296
Locus At3g56580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20962701
Primers used for PCR 44et - LBb3, show primer details
   
Sequence Name 22-K111273-SALK_024296C-44et
GenBank Accession LN484912 [GenBank]
Sequencing primer gene specific primer, 44et: 5'-TAATTCGATTTTCTCTCCATTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K111273-SALK_024296C-44et
TTTTTTCTGCTCAATGTGGTTGACTATAGAGTTTCTGAGTTCATTATTTGGTCATTTTTT
AATTGAGTTGGCTTGATTAAAGAAGAATTTATTTGTTAATCAGCTAGATTAGATTTGAAG
ATTGTAGTAGATTAGTATGATGTGAATGTTCTAATCTGGGAATAAAGGGTCTAGTGTTGA
GGGAAACATGAGATTTTTCATAATTGGGTGAAATATGTGTTGTAAAGTTTGGTCTTTTAT
TTGCTTAATTATGACTTGTGGTTCCATTGACTCTTGTAGTATGAACTAAGCTCTGAGGTA
AGCTTGGGAAGTAGGGATATGGCCAAAATCTCTTTGGTTTACCAACTTAGCAAAAAAGTT
AGCAGGAGTTGGTGATCAGTTCTCCTACCGGCTTTTGCAGGCTAATAACTAATACCTGAC
TGTAAAATCTTCCAAGGTTTCAACCACACGGGGACCTTG
   
Sequence Name 06-K111272-SALK_024296C-R210
GenBank Accession LN484426 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K111272-SALK_024296C-R210
CATTTGTTTCACCACAAACCCTGTGAGCTCTACTCGGTCCTATATCGATTTCCTCAACAA
ACCCTCCTCCACAATATGAACAGACAGCATCTCGAGCTCGAAGCCAAACAGCACGTTGAC
ATCTGTGACACCAATGAGTATTCCGAATACTTGACATCCTTTACAATAACAAGAGAAACA
AGGTCCCTGTGTGGTTGAAACCTTGGAAGATTTTACAGTCAGGTATTAGTTATTAGCCTG
CAAAAGCCGGTAGGAGAACTGATCACCAACTCCTGCTAACTTTTTTGCTAAGTTGGTAAA
CCAAAGAGATTTTGGCCATATCCCTACTTCCCAAGCTTACCTCAGAGCTTAGTTCATACT
ACAAGAGTCAATGGAACCACAAGTCATAATTAAGCAAATAAAAGACCAAACTTTACAACA
CATATTTCACCCAATTATGAAAAATCTCATGTTTCCCTCAACACTAGACCCTTTATTCCC
AGATTAGAACATTCACATCATACTAATCTACTACAATCTTCAAATCTAATCTAGCTGATT
AACAAATAAATTCTTCTTTAATCAAGCCAACTCAATTAAAAAATGACCAAATAATGAACT
CAGAAACTCTATAGTTCAACCACATTTGAGCTAGAAAAAAAAACTCTAAATGAGCCATCA
GGCGATCCAGCAATGGAGAGAATTCCGAAT

Last Updated on 06.12.2018 16:11