Sequences from confirmation amplicon


Confirmation sequences for line SALK_024636c and hit At5g12010 are shown.
Line ID SALK_024636c
Locus At5g12010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3878853
Primers used for PCR 63kh - LBb3, show primer details
   
Sequence Name 02-K114130-SALK_024636C-R210
GenBank Accession LN484313 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114130-SALK_024636C-R210
TGATATATTGCGGTTGCCAATTTGTTTATTCCGTCAGCGAGTCGCGGTTTGTATATGGAG
ATTAGCCACCGGTGAGCCACTTCGTCTTGTCTCCAAGAAGTTTGGTTTAGGAATCTCAAC
TTGTCACAAGCTTGTTCTTGAGGTATGTAAAGCTATTAAGGACGTTTTAATGCCTAAGTA
TCTTCAATGGCCAGATGATGAATCTCTTAGAAACATTAGAGAACGTTTCGAATCGGTTTC
GGGTATTCCAAACGTTGTTGGCTCTATGTATACTACTCACATTCCGATTATAGCTCCTAA
GATTAGTGTAGCCTCTTATTTCAATAAGAGGCATACTGAGAGGAACCAGAAGACGTCGTA
TTCGATTACAATTCAAGCCGTTGTGAACCCGAAAGGTGTTTTTACTGACTTGTGCATCGG
ATGGCCCGGGTCAATGCCGGATGATAAGGTGTTAGAGAAGTCATTGTTGTATCAAAGAGC
TAACATGTGGAGGTT
   
Sequence Name 12-K114131-SALK_024636C-63kh
GenBank Accession LN484617 [GenBank]
Sequencing primer gene specific primer, 63kh: 5'-AAACCTCCATTGTTAGCTCTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114131-SALK_024636C-63kh
CCGGCATTGACCCGGGCCATCCGATGCACAAGTCAGTAAAAACACCTTTCGGGTTCACAA
CGGCTTGAATTGTAATCGAATACGACGTCTTCTGGTTCCTCTCAGTATGCCTCTTATTGA
AATAAGAGGCTACACTAATCTTAGGAGCTATAATCGGAATGTGAGTAGTATACATAGAGC
CAACAACGTTTGGAATACCCGAAACCGATTCGAAACGTTCTCTAATGTTTCTAAGAGATT
CATCATCTGGCCATTGAAGATACTTAGGCATTAAAACGTCCTTAATAGCTTTACATACCT
CAAGAACAAGCTTGTGACAAGTTGAGATTCCTAAACCAAACTTCTTGGAGACAAGACGAA
GTGGCTCACCGGTGGCTAATCTCCATATACAAACCGCGACTCGCTGACGGAATAAACAAA
TTGGCAACCGCAATATATCACATTGACGCTNAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11