Sequences from confirmation amplicon


Confirmation sequences for line SALK_024656 and hit At5g51590 are shown.
Line ID SALK_024656
Locus At5g51590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20958427
Primers used for PCR 12rb - LBb3, show primer details
   
Sequence Name 69-K116055-SALK_024656C-12rb
GenBank Accession LN485986 [GenBank]
Sequencing primer gene specific primer, 12rb: 5'-AATTATCGAAAACATACCTCACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K116055-SALK_024656C-12rb
CTTGGACTGACAATTTCAGCAGGTCCTCCTCCACCTGCAAATTAATCATCAAATGAATCA
AGCTTAACAGATAAAACAAACGAAATCTGATCAAGAAAACTAACAAATTGCTAAAACCAA
AACAGTAAAGAGTTCAAGTTAAATCCTTAAGTAATAAGACCAAAGCCTACAGATTCCTCG
ATATATATACTCTTAATCACACTGCTTATGTCACTATCATCAAAGAATCCGAACTAGTTT
CCCTAAATTGCCTTCTTCTTCTGGGACTTTTCCTTTAAATTCAACTCTTTTTTCACACAG
AATCCTCCAGTTTACTGGACAGTAACATTCACTAGCTTTATAAGACAAAACCTTTGTCCT
TATTCCAAAATTTTTGCAAGTCAAATAGATCTATTAAAAGCTATCTATTGATTCTTATAC
AGAGAGGCCAATGTACACACACAGAGATTCCTAAATTCGAACAGGAAAGGAGAAAAAGAC
ATTACCAGAAGTAGGAGTGTTGTTGTTAAATTCGAACATCTGAGGATTCTTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 42-K116054-SALK_024656C-R210
GenBank Accession LN485335 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116054-SALK_024656C-R210
TCTTTTTCTCCTTTCCTGTTCGAAATTAAGACTCTCTGTGTGTGTACATTGCCCTCTCTG
TATAAGAATCAATAGATAGCTTTTAATATATCTATTTGACTTGCAAAAATTTTGGAATAA
GGACAAAGGTTTTGTCTTATAAAGCTAGTGAATGTTACTGTCCAGTAAACTGGAGGATTC
TGTGTGAAAAAAGAGTTGAATTTAAAGGAAAAGTCCCAGAAGAAGAAGGCAATTTAGGGA
AACTAGTTCGGATTCTTTGATGATAGTGACATAAGCAGTGTGATTAAGAGTATATATATC
GAGGAATCTGTAGGCTTTGGTCTTATTACTTAAGGATTTAACTTGAACTCTTTACTGTTT
TGGTTTTAGCAATTTGTTAGTTTTCTTGATCAGATTTCGTTTGTTTTATCTGTTAAGCTT
GATTCATTTGATGATTAATTTGCAGGTGGAGGAGGACCTGCTGAAATTGTCAGTCCAAGT
TTTACACCTCATGTGCTCACAGTAAATGCCGGTGAGGTATGTTTTCGATAATT

Last Updated on 06.12.2018 16:11