Sequences from confirmation amplicon


Confirmation sequences for line SALK_024766c and hit At5g64850 are shown.
Line ID SALK_024766c
Locus At5g64850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25922830
Primers used for PCR 23tg - LBb3, show primer details
   
Sequence Name 27-K117180-SALK_024766C-23tg
GenBank Accession LN485070 [GenBank]
Sequencing primer gene specific primer, 23tg: 5'-GCCAATTGGTTGTAATCCATATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117180-SALK_024766C-23tg
CGTACTTCATTGTTATATATGCAAACATGATATTTGAATACTTTTCGAGTAAACACCATT
CATTTTTTTTTCATTAATCTAAAAACGCATTATCGTAATGTATCACAAATCTGCCCTACA
TTACACAATAATGAAAATTATCAGTGTCTAAATACACCAAACAAAAATTACAAAAAATAT
ATATTACTACACTTGACATTTAGCAAACATAGTTAGATACACAGTAAACAACAACAGACA
AATTTTAATTGTGATGCAGCCGAAGAAACACATGTGGAAAACAAGGTGGCACTACCATTT
CTCATTTCTGTCTTCAAATATCACCCCTTGTTGTTATTATCATAAATAAAAGTAAACACC
TTTAAGCTTTAACGCAACAGTTGAAGCAGCTGAAGATTCTTCTCCTCCTCTGTTCCAAAG
TCATTTACAAACAAGATTAAGTATGTTTTATCCAAAAAAAAGAACAACATTATTTATGTC
ACTTCACATGGTAGGTACAAAATAAAGGATTATGCTTTACTCAGATTGAAAGAAAGAATC
CATTATAATAACTTTGTAGGGTAATGTACTCTGTAGGGTAATAACTTTGTAAACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 06-K117179-SALK_024766C-R210
GenBank Accession LN484439 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117179-SALK_024766C-R210
AAGTTATTACCCTACAGAGTACATTACCCTACAAAGTTATTATAATGGATTCTTTCTTTC
AATCTGAGTAAAGCATAATCCTTTATTTTGTACCTACCATGTGAAGTGACATAAATAATG
TTGTTCTTTTTTTTGGATAAAACATACTTAATCTTGTTTGTAAATGACTTTGGAACAGAG
GAGGAGAAGAATCTTCAGCTGCTTCAACTGTTGCGTTAAAGCTTAAAGGTGTTTACTTTT
ATTTATGATAATAACAACAAGGGGTGATATTTGAAGACAGAAATGAGAAATGGTAGTGCC
ACCTTGTTTTCCACATGTGTTTCTTCGGCTGCATCACAATTAAAATTTGTCTGTTGTTGT
TTACTGTGTATCTAACTATGTTTGCTAAATGTCAAGTGTAGTAATATATATTTTTTGTAA
TTTTTGTTTGGTGTATTTAGACACTGATAATTTTCATTATTGTGTAATGTAGGGCAGATT
TGTGATACATTACGATAATGCGTTTTTAGATTAATGAAAAAAAAATGAATGGTGTTTACT
CGAAAAGTATTCAAATATCATGTTTGCATATATAACAATGAAGTTACGTTTAATAAAATC
ATCCTCTCGAAGTTGTAGAGATATGGATTACAACCAATTGGC

Last Updated on 06.12.2018 16:11