Sequences from confirmation amplicon


Confirmation sequences for line SALK_025156c and hit At3g04680 are shown.
Line ID SALK_025156c
Locus At3g04680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1270261
Primers used for PCR 45fm - LBb3, show primer details
   
Sequence Name 11-K111624-SALK_025156C-45fm
GenBank Accession LN484582 [GenBank]
Sequencing primer gene specific primer, 45fm: 5'-CAAAGAGGTCCGTAAAAGATCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K111624-SALK_025156C-45fm
TCATCTCAATCTTTTATATCCCTTTTTTATGCCTTTGGAGGTGTAACTCAGAACTCAAAA
ACTGAATTTTCCAGGAATATTTTTACGGTTTATCGAAAGAACTCTCTCCGTATGCCAATA
CATCGAGTTTCAGTGATCTGCAAGTGTTCCGAATTGGTGGTGGACCACAAGCGCCAAAAT
CGGCTCTTCCAGCCGGGTCTACCTCGGTTTCAAATCCGTTGCGGGTGACGCCTGTAAATA
TCGATGATCGGGATCTGCTGCATTCTGTTCTTGCTGTCTCATACGCAGAAGAACCAGACC
AGATCATCTCAAGGTAATCTCTAAAAGTTCTTGCTGTTTCCAGGATATATTGTGGTGTAA
ACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCGAATCNGGCAAAA
   
Sequence Name 03-K111623-SALK_025156C-R210
GenBank Accession LN484335 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K111623-SALK_025156C-R210
GTCATTTGTTTACCCACAATATATCCTGGAAACGCAAGAACTTTTAGAGATTACCTTGCG
ATGATCTGGTCTGGTTCTTCTGCGTATGAGACAGCAAGAACAGAATGCAGCAGATCCCGA
TCATCGATATTTACAGGCGTCACCCGCAACGGATTTGAAACCGAGGTAGACCCGGCTGGA
AGAGCCGATTTTGGCGCTTGTGGTCCACCACCAATTCGGAACACTTGCAGATCACTGAAA
CTCGATGTATTGGCATACGGAGAGAGTTCTTTCGATAAACCGTAAAAATATTCCTGGAAA
ATTCAGTTTTTGAGTTCTGAGTTACACCTCCAAAGGCATGAAAAAAGGGATATTAAAAGA
TTTGAGATTGAAAATTTTACCTGAATCTTAAAGTTCCTTGATCTTTTACGGACCTCTTTG

Last Updated on 06.12.2018 16:11