Sequences from confirmation amplicon


Confirmation sequences for line SALK_025428c and hit At5g27760 are shown.
Line ID SALK_025428c
Locus At5g27760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9830660
Primers used for PCR 34vi - LBb3, show primer details
   
Sequence Name 43-K117945-SALK_025428C-34vi
GenBank Accession LN485360 [GenBank]
Sequencing primer gene specific primer, 34vi: 5'-TTAAATTTTGATGGCTTTGGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117945-SALK_025428C-34vi
AATTAGCGAATGTTGAAAGACAATTTTTTACACTTTATTAAGACACGTCAGCAAAATGAA
GCGCAAGTTAGGAAATTGGCTTTTACTTCTTCCTTGAGAAGTTTCCGACTCGGCCGCTTT
AAAACCAACGCCCGACGCTCATTTCTTCTCTCCATTTTTTTTTGCTCATCTTCTTCAACA
ATGGCGGAACCAAAGACAAAAGTTGCAGAAATCAGGGAATGGATCATCGAACATAAGCTT
CGTACCGTTGGTAATCTCACTCTTCCTCCTCTTATTCCCTCTTTGTTCATCCTCGAACTA
ATTTTCTCATAGGTTGTTTTGGCGTAACAATAATACACGAATCGAGTTTCGTATTGGTTC
ATCGTTATTATAGATCTTGTTTATACCGTATTTTTCTGAATAATTCCTCAGATCCGAAGT
TTTTTTCGATCTGAATAATCTCAATTTTGACGCTAAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCAAAACGGGGC
   
Sequence Name 08-K117944-SALK_025428C-R210
GenBank Accession LN484504 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117944-SALK_025428C-R210
ATTGAGATTATTAGATCGAAAAAACTTCGGATCTGAGGAATTATTCAGAAAAATACGGTA
TAAACAAGATCTATAATAACGATGAACCAATACGAAACTCGATTCGTGTATTATTGTTAC
GCCAAAACAACCTATGAGAAAATTAGTTCGAGGATGAACAAAGAGGGAATAAGAGGAGGA
AGAGTGAGATTACCAACGGTACGAAGCTTATGTTCGATGATCCATTCCCTGATTTCTGCA
ACTTTTGTCTTTGGTTCCGCCATTGTTGAAGAAGATGAGCAAAAAAAAATGGAGAGAAGA
AATGAGCGTCGGGCGTTGGTTTTAAAGCGGCCGAGTCGGAAACTTCTCAAGGAAGAAGTA
AAAGCCAATTTCCTAACTTGCGCTTCATTTTGCTGACGTGTCTTAATAAAGTGTAAAAAA
TTGGTCTTTCAACATTCGCTAATTTTTGACTTTTTTTCAACGCTAAATTGATTCCAAAGC
CATCAAAATTTA

Last Updated on 06.12.2018 16:11