Sequences from confirmation amplicon


Confirmation sequences for line SALK_025557c and hit At3g03480 are shown.
Line ID SALK_025557c
Locus At3g03480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:828717
Primers used for PCR 46fm - LBb3, show primer details
   
Sequence Name 12-K111624-SALK_025557C-46fm
GenBank Accession LN484610 [GenBank]
Sequencing primer gene specific primer, 46fm: 5'-TTTCTGAAATCACTATGCGAGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K111624-SALK_025557C-46fm
GACCGACACTTGTTGACTGTGAGTGCTTCGGAGGCGCGTGTGACCCACACACACCGTGAG
TACGATGATCAGGTGGGAATCGATGTGGTCGCCACTGGACACCCTTTGGTTAGCCGGTCT
TTCTTTTTCAGAGCAGAAGAGATCTCCGCTATAAGGAAACTCCTTCCGCCTGATCTACAC
AATACCTCATTTGAAGCTTTGTCGTCATTTTTGTGGCGTTGTCGGACTATTGCGTTGAAT
CCTGACCCCAACACTGAGATGCGTCTAACTTGCATCATCAACTCGCGTTCCAAGCTTAGA
AACCCACCGCTAGAGCCTGGTTACTACGGAAATGTGTTTGTCATTCCCGCGGCAATAGCG
ACAGCAAGAGATCTAATAGAGAAACCGCTTGAGTTTGCTTTGAGGTTGATACAAGAAACG
AAGTCAAGTGTAAACAAATATACAAATTGACGCTTAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNGGCAAAA
   
Sequence Name 04-K111623-SALK_025557C-R210
GenBank Accession LN484365 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K111623-SALK_025557C-R210
GTCATTTGATATTTGTTTACCTTGACTTCGTTTCTTGTATCAACCTCAAAGCAAACTCAA
GCGGTTTCTCTATTAGATCTCTTGCTGTCGCTATTGCCGCGGGAATGACAAACACATTTC
CGTAGTAACCAGGCTCTAGCGGTGGGTTTCTAAGCTTGGAACGCGAGTTGATGATGCAAG
TTAGACGCATCTCAGTGTTGGGGTCAGGATTCAACGCAATAGTCCGACAACGCCACAAAA
ATGACGACAAAGCTTCAAATGAGGTATTGTGTAGATCAGGCGGAAGGAGTTTCCTTATAG
CGGAGATCTCTTCTGCTCTGAAAAAGAAAGACCGGCTAACCAAAGGGTGTCCAGTGGCGA
CCACATCGATTCCCACCTGATCATCGTACTCACGGTGTGTGTGGGTCACACGCGCCTCCG
AAGCACTCACAGTCAACAAGTGTCGGTTCCATACGGGTGGAACCGAGGGTGCGTGAAGCC
CACACGCCAACTCGCATAGTGATTTCAGAA

Last Updated on 06.12.2018 16:11