Sequences from confirmation amplicon


Confirmation sequences for line SALK_026061c and hit At1g03050 are shown.
Line ID SALK_026061c
Locus At1g03050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:707710
Primers used for PCR 23di - LBb3, show primer details
   
Sequence Name 70-K110311-SALK_026061C-23di
GenBank Accession LN486000 [GenBank]
Sequencing primer gene specific primer, 23di: 5'-AACGCAGAGTAGTCCCAAGAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K110311-SALK_026061C-23di
TATTGAGAACCTTGTTCCCGGCGAGTGGCGAAGAAGATCTCTTGCTCGTAGGCTTGATCT
CCTTCTCCTAAGAGCCTTTGGATCAAAATCAAGGTTTTGAGGGCAACGGTCCAGCATTTG
GTTTTATTAAGGCGTCTCGAAAGCGTGCTAACGCAAGCGTTGATGTAACTGCGTGAGTAA
GAAGTTAGGCTAAGAATCTCTCTAATGTATTTCTCTTCTGCTGGAAACTCTTCGTGACGA
GTGGCTTTGACGATGGCTACGTCAAGTTCAGATAAAGAAGCGCTTCGACCATTGACTTTG
GCTAGACCGACGCTGGTTTGGTCCTTTACGGCTCCTATGGCTCGTTTAAACTTACTCGAA
CCCATTTTTATTCTGATTAGTATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 60-K110310-SALK_026061C-R210
GenBank Accession LN485738 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K110310-SALK_026061C-R210
GCGTCATTTGTTTACCCACAATATATACTAATCAGAATAAAAATGGGTTCGAGTAAGTTT
AAACGAGCCATAGGAGCCGTAAAGGACCAAACCAGCGTCGGTCTAGCCAAAGTCAATGGT
CGAAGCGCTTCTTTATCTGAACTTGACGTAGCCATCGTCAAAGCCACTCGTCACGAAGAG
TTTCCAGCAGAAGAGAAATACATTAGAGAGATTCTTAGCCTAACTTCTTACTCACGCAGT
TACATCAACGCTTGCGTTAGCACGCTTTCGAGACGCCTTAATAAAACCAAATGCTGGACC
GTTGCCCTCAAAACCTTGATTTTGATCCAAAGGCTCTTAGGAGAAGGAGATCAAGCCTAC
GAGCAAGAGATCTTCTTCGCCACTCGCCGTGGAACAAGGCTTCTCAATATGTCTGATTTT
AGGGATGTTTCGAGGTCTAATTCTTGGGACTACTCTGCGT

Last Updated on 06.12.2018 16:11