Sequences from confirmation amplicon


Confirmation sequences for line SALK_026686 and hit At1g69090 are shown.
Line ID SALK_026686
Locus At1g69090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25978086
Primers used for PCR 53wx - LBb3, show primer details
   
Sequence Name 30-K118859-SALK_026686C-53wx
GenBank Accession LN485140 [GenBank]
Sequencing primer gene specific primer, 53wx: 5'-TTGGATTAGGATAGGCTCCTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118859-SALK_026686C-53wx
GAGTGAAAAAAGAAAAGCTAATATCATCTCCCACACTTGAAAGCTTGACGGATAAGATAA
TCCGAAAGATGGCCTCACCAACGTTAGCCTTAGCCCAATCTCCTCCTCCGAAATCTCCAG
CTGTTTCAGTCTCTCAAAGAAATCCTCATTGCTGGTCCAAGCTTCCTCTAGATCTCATGC
AATTGGTTTTCGAACGCCTTGCATTTTTGGATTTCGAACGAGCTAAATCGGTTTGTTCAT
CTTGGCAATTTGGTTCAAAACAATCTAAGCCAAACAATCAGATCCCTTGGATGATTCTAT
TCCCAACAGACAAGAATTATTGTCTCTTGTTCAACCCTGAAGATAAAGAAAAACTCTACA
AAACTCAACATCTCGGTGACGATTTTGCAAAGAGTATTGTTTTGGCGACTTATAGAAGTT
GGCTTTTGATGCAACCTCGTTACGAAGAGTTAGAAGATCAAACTTTAGATCAAGAGTTTC
ATCTATATATTAAAGATCTTCTAACCTGCGAGAGGATCAATCTACCGGCTTTCGAATCAG
ATATTTTTGGACTTAGCCATCCGATATTATGGATAGATGACAAAACCAAAGACTACCTAG
TTATAGGAACGATTAATCGAGAAACTATGGTTTCTTTCAAAAATGGAGATAACTCGTGGA
AAAAATTCCCCGAGTTGCCAAAATCGTCATGTACCGACATGTGTTTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAAATCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
T
   
Sequence Name 06-K118858-SALK_026686C-R210
GenBank Accession LN484444 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118858-SALK_026686C-R210
TGACGATTTTGGCAACTCGGGGAATTTTTTCCACGAGTTATCTCCATTTTTGAAAGAAAC
CATAGTTTCTCGATTAATCGTTCCTATAACTAGGTAGTCTTTGGTTTTGTCATCTATCCA
TAATATCGGATGGCTAAGTCCAAAAATATCTGATTCGAAAGCCGGTAGATTGATCCTCTC
GCAGGTTAGAAGATCTTTAATATATAGATGAAACTCTTGATCTAAAGTTTGATCTTCTAA
CTCTTCGTAACGAGGTTGCATCAAAAGCCAACTTCTATAAGTCGCCAAAACAATACTCTT
TGCAAAATCGTCACCGAGATGTTGAGTTTTGTAGAGTTTTTCTTTATCTTCAGGGTTGAA
CAAGAGACAATAATTCTTGTCTGTTGGGAATAGAATCATCCAAGGGATCTGATTGTTTGG
CTTAGATTGTTTTGAACCAAATTGCCAAGATGAACAAACCGATTTAGCTCGTTCGAAATC
CAAAAATGCAAGGCGTTCGAAAACCAATTGCATGAGATCTAGAGGAAGCTTGGACCAGCA
ATGAGGATTTCTTTGAGAGACTGAAACAGCTGGAGATTTCGGAGGAGGAGATTGGGCTAA
GGCTAACGTTGGTGAGGCCATCTTTCGGATTATCTTATCCGTCAAGCTTTCAAGTGTGGG
AGATGATATTAGCTTTTTCTTTTTTTCACTCTTTGAGAGAAAGTTCATATTAAGAGATTA
AAAACTAAAGGAGCCTATCCTAATCCAA

Last Updated on 06.12.2018 16:11