Sequences from confirmation amplicon


Confirmation sequences for line SALK_026853 and hit At4g01970 are shown.
Line ID SALK_026853
Locus At4g01970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:854891
Primers used for PCR 18ea - LBb3, show primer details
   
Sequence Name 59-K110715-SALK_026853C-18ea
GenBank Accession LN485712 [GenBank]
Sequencing primer gene specific primer, 18ea: 5'-AGACCTTGGAGTCACTAGCAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K110715-SALK_026853C-18ea
GACTGCTAGCTTACTCGATGGTCTGACCGAATCCTGATCAAGAATTTTAATGGAACTGAT
GTCATTGCGAGTATGCAACAGTGCAATGAGTTCTTCTTCCTTGCCACAAAGCAAATCTCT
ATTGGCAGAGTTGGTAATATATATAAAGCTAAAGCCGTTTCTTTTGATTCCACTAGTGTT
TGAGCATTAACAAAAGTTTTGAATTTTGCTCAGGTGATGATTTTTGGTGGCAAGACCCAT
ATGGTGACCCACAAGGAGTATATTGGCTACAAGGAGTTCACATGATTCACTGTTCTTACA
ACAGTATTTGGATGGGTCAAATGATTCAACCAGATTGGGACATGTTCCAATCAGACCATG
TCTGTGCCGAGTACCACGCCGCATCTCGAGCCATTTGCGGTGGGCCTGTCTATCTCAGTG
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAATCNGGCAAAA
   
Sequence Name 43-K110714-SALK_026853C-R210
GenBank Accession LN485352 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K110714-SALK_026853C-R210
GTCATTTGTTACACTGAGATGACAGGCCCACCGCAAATGGCTCGAGATGCGGCGTGGTAC
TCGGCACAGACATGGTCTGATTGGAACATGTCCCAATCTGGTTGAATCATTTGACCCATC
CAAATACTGTTGTAAGAACAGTGAATCATGTGAACTCCTTGTAGCCAATATACTCCTTGT
GGGTCACCATATGGGTCTTGCCACCAAAAATCATCACCTGAGCAAAATTCAAAACTTTTG
TTAATGCTCAAACACTAGTGGAATCAAAAGAAACGGCTTTAGCTTTATATATATTACCAA
CTCTGCCAATAGAGATTTGCTTTGTGGCAAGGAAGAAGAACTCATTGCACTGTTGCATAC
TCGCAATGACATCAGTTCCATTAAAATTCTTGATCATGGATTCGGTCAGACCATCGTAGT
AAGCTTTAGCAAGCTCCACTCGTCCTCCATGTTCTTCTGCTAGTGACTCCAAGGTC

Last Updated on 06.12.2018 16:11