Sequences from confirmation amplicon


Confirmation sequences for line SALK_026969c and hit At5g47730 are shown.
Line ID SALK_026969c
Locus At5g47730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19336766
Primers used for PCR 19ea - LBb3, show primer details
   
Sequence Name 58-K111283-SALK_026969C-19ea
GenBank Accession LN485687 [GenBank]
Sequencing primer gene specific primer, 19ea: 5'-TGAAATCCCAAACTAATAACACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K111283-SALK_026969C-19ea
AAGTATTAAACGTTTCTAAAAAAAGACAACTTTGAATATTTGACCCAAAAAGCAGTAAAG
AAAAAACAAAAAGAGACCAACTTTGAATACCATTATTTATCTTTTGACAAGGCTCACCAA
ATCAGCTACCAAGTTCATACATGGTACAAAGCTTTAGCAAGTTGTCGAATTTCATCCTAC
AATTGAATTTTCTCTTTACTATTTCAAAGTTTGAATGCTCCTCTGTTTTCTTCGTTGGTT
CATTGAGAAAAAAGGAACAGACTTTTTACTTGTGCCATTGACCAAGAACCACAAAGAGGA
GGAAATCAAATGGTTTTTGGGAGTTTGATGGATTGATTGATTATTCAGCAAATCCCACAG
AAGAAATCAGAAGCTGCTTCTTATTTTTTGGTCACGACCAGTCTTAATCAAAGTAGTTCG
TCTCCTACTTGTTATTAAAAGTGTATATTTCTGTTGGGAGTTTCTGTTCTCCTACCTCAC
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTC
   
Sequence Name 42-K111282-SALK_026969C-R210
GenBank Accession LN485331 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K111282-SALK_026969C-R210
AAACTCCCAACAGAAATATACACTTTTAATAACAAGTAGGAGACGAACTACTTTGATTAA
GACTGGTCGTGACCAAAAAATAAGAAGCAGCTTCTGATTTCTTCTGTGGGATTTGCTGAA
TAATCAATCAATCCATCAAACTCCCAAAAACCATTTGATTTCCTCCTCTTTGTGGTTCTT
GGTCAATGGCACAAGTAAAAAGTCTGTTCCTTTTTTCTCAATGAACCAACGAAGAAAACA
GAGGAGCATTCAAACTTTGAAATAGTAAAGAGAAAATTCAATTGTAGGATGAAATTCGAC
AACTTGCTAAAGCTTTGTACCATGTATGAACTTGGTAGCTGATTTGGTGAGCCTTGTCAA
AAGATAAATAATGGTATTCAAAGTTGGTCTCTTTTTGTTTTTTCTTTACTGCTTTTTGGG
TCAAATATTCAAAGTTGTCTTTTTTTTTAGAAACGTTTTAATTACTTTTTAAGTGGTTTA
TTAGAGGGTGTTATTAGTTTGGGGATTTCA

Last Updated on 06.12.2018 16:11