Sequences from confirmation amplicon


Confirmation sequences for line SALK_027235c and hit At2g43490 are shown.
Line ID SALK_027235c
Locus At2g43490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18057520
Primers used for PCR 15td - LBb3, show primer details
   
Sequence Name 72-K116811-SALK_027235C-15td
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 15td: 5'-GGGGTATGTTTTCACTTTGGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K116811-SALK_027235C-15td
CCCGTTTGATGGCCGTTTCTAATTTTTTCCGCTACCCCCCC
   
Sequence Name 72-K116810-SALK_027235C-R210
GenBank Accession LN486063 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K116810-SALK_027235C-R210
CGCATTGCTTGAGCAATCATTGTATCGCTTCCTGCGTGAAAATTCCAATAACGCTTGTAA
GCTTTTTGATTAAACTTAACTTGCTATTAGCACTCTTTCTCACTCTAAGCTGTGATCAAA
CAGTCTAACCTTCGAGCTACCCTGAGTTGATTACGGTACTCAGAGGTGCTGCTCAACGCA
TAACAACCAAGGAGAAATTCCCAAACTTCAGCTCTAATAGATGGGTCGATTCCCTAATGA
AAAACATCATACATTATACCACTGTACTGAAAGAATAATCAAAAGCGTTACAAGTTCTTC
TACCAAAGTGAANACATACCCC

Last Updated on 06.12.2018 16:11