Sequences from confirmation amplicon


Confirmation sequences for line SALK_027974c and hit At4g25560 are shown.
Line ID SALK_027974c
Locus At4g25560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13053441
Primers used for PCR 33um - LBb3, show primer details
   
Sequence Name 05-K117668-SALK_027974C-R210
GenBank Accession LN484411 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117668-SALK_027974C-R210
TTTGTTTACCCACAACAACAGCAACTGGACTCTTAACGACATTGTGTTTGGTTCCAAATG
TAAGAAGCAGGAGCATCATATTTATAGAGAGGCTTCAGATTGTAATTCTTCTGCTGAATT
CTTTTCTCCATCAACAACGACGTAAATTGCGTTTATTGTAATGTAAATCAAATTTCTAAG
GCAAAACCGGAAAAAAAATGTTTTGTTTTTTGTTTTGTCTTAAATGCTTTGACACCAATA
AGGCAAATAGCATATATGGTTAAAGGAATGGCAATATGAAAGAAAAGAAAAGAAAAGAAA
ATGATTTAAAAGATTCATCAATATCATTGAAGATTTTACACACCAGAAGTAGCAAAGCCG
TAACACACAAAGCTTTTCGCTACAAATGCATCATACATAAATGGAGTTTTGAGAGTACAA
GGGGCGATACAGATGTAACGTGGAATGTAGAAAGTGTTTTATATAGCAGAATAACTGAAG
TCAACACTATCATCATTGGAGACGGAAGGAGAAGGAGAAGGAGACTCAGCAGAAGCAGTG
AGTACCACAAAGGCACCAAAGAGAATAGTGATAATG
   
Sequence Name 42-K117669-SALK_027974C-33um
GenBank Accession LN485339 [GenBank]
Sequencing primer gene specific primer, 33um: 5'-ACTTCACGGCCATTATCACTATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117669-SALK_027974C-33um
CTTCTCCTTCTCCTTCCGTCTCCAATGATGATAGTGTTGACTTCAGTTATTCTGCTATAT
AAAACACTTTCTACATTCCACGTTACATCTGTATCGCCCCTTGTACTCTCAAAACTCCAT
TTATGTATGATGCATTTGTAGCGAAAAGCTTTGTGTGTTACGGCTTTGCTACTTCTGGTG
TGTAAAATCTTCAATGATATTGATGAATCTTTTAAATCATTTTCTTTTCTTTTCTTTTCT
TTCATATTGCCATTCCTTTAACCATATATGCTATTTGCCTTATTGGTGTCAAAGCATTTA
AGACAAAACAAAAAACAAAACATTTTTTTTCCGGTTTTGCCTTAGAAATTTGATTTACAT
TACAATAAACGCAATTTACGTCGTTGTTGATGGAGAAAAGAATTCAGCAGAAGAATTACA
ATCTGAAGCCTCTCTATAAATATGATGCTCCTGCTTCTTACATTTGGAACCAAACACAAT
GTCGTTAAGAGTCCAGTTGCTGTTGTTGTGGTGTAAACAAATTGACGCTTAGACAACTTA
ATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACG
GGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
CTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11